Assay Detail

CHEMBL2401270

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Functional

Inhibition of TGF-beta-induced PTHrP secretion in human MDA-MB-231 cells treated 4 hrs before TGF-beta challenge measured after 24 hrs by radioimmunoassay

ChEMBL ↗

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Target

MDA-MB-231 (CHEMBL400) ↗

Open DB target context

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MDA-MB-231 (CHEMBL400) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target MDA-MB-231

CHEMBL400

Anchored from Assay target.

Protein identity MDA-MB-231

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Homo sapiens
Cell Type	MDA-MB-231
Confidence	1 — Organism
Curated By	Autocuration

Target

MDA-MB-231 (CHEMBL400) ↗

Type	CELL-LINE
Organism	Homo sapiens

Publication

Curcuminoids block TGF-β signaling in human breast cancer cells and limit osteolysis in a murine model of breast cancer bone metastasis.

Wright LE, Frye JB, Lukefahr AL, Timmermann BN, Mohammad KS, Guise TA, Funk JL.
J Nat Prod (2013) 76 : 316 -321
PubMed 23145932 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	3	4.62	4.62
MEC	3	-	-
Inhibition	3	-	-

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL105350	BISDEMETHOXYCURCUMIN	—	3	4.62
CHEMBL179512	DEMETHOXYCURCUMIN	—	3	4.62
CHEMBL140	CURCUMIN	3.0	3	4.62

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL105350	BISDEMETHOXYCURCUMIN	IC50	=	24000.0	nM	4.62
CHEMBL179512	DEMETHOXYCURCUMIN	IC50	=	24000.0	nM	4.62
CHEMBL140	CURCUMIN	IC50	=	24000.0	nM	4.62
CHEMBL105350	BISDEMETHOXYCURCUMIN	MEC	=	13.0	uM	-
CHEMBL179512	DEMETHOXYCURCUMIN	MEC	=	13.0	uM	-
CHEMBL140	CURCUMIN	MEC	=	13.0	uM	-
CHEMBL105350	BISDEMETHOXYCURCUMIN	Inhibition		-	%	-
CHEMBL179512	DEMETHOXYCURCUMIN	Inhibition		-	%	-
CHEMBL140	CURCUMIN	Inhibition		-	%	-