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Assay Detail

CHEMBL2401298

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma-secretase in human IMR32 cell membrane using Notch as substrate after 2 hrs by ELISA
17
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type IMR-32
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Discovery of a novel [3.2.1] benzo fused bicyclic sulfonamide-pyrazoles as potent, selective and efficacious γ-secretase inhibitors.
Ye XM, Konradi AW, Sun M, Yuan S, Aubele DL, Dappen M, Dressen D, Garofalo AW, Jagodzinski JJ, Latimer L, Probst GD, Sham HL, Wone D, Xu YZ, Ness D, Brigham E, Kwong GT, Willtis C, Tonn G, Goldbach E, Quinn KP, Zhang HH, Sauer JM, Bova M, Basi GS.
Bioorg Med Chem Lett (2013) 23 : 996 -1000
PubMed 23312944 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.03 8.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1090771 AVAGACESTAT 2.0 1 8.82
CHEMBL2396778 1 8.28
CHEMBL463981 BEGACESTAT 2.0 1 7.98
CHEMBL2396964 1 7.92
CHEMBL2396959 1 7.85
CHEMBL2396953 1 7.75
CHEMBL2396952 2 7.44
CHEMBL2396960 1 7.35
CHEMBL2396963 1 7.31
CHEMBL2396958 1 6.93
CHEMBL2396965 1 6.90
CHEMBL2396961 1 6.78
CHEMBL2396966 1 6.72
CHEMBL2396951 1 6.18
CHEMBL2396967 1 5.55
CHEMBL2396962 1 5.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1090771 AVAGACESTAT IC50 = 1.5 nM 8.82
CHEMBL2396778 IC50 = 5.3 nM 8.28
CHEMBL463981 BEGACESTAT IC50 = 10.5 nM 7.98
CHEMBL2396964 IC50 = 12.0 nM 7.92
CHEMBL2396959 IC50 = 14.0 nM 7.85
CHEMBL2396953 IC50 = 18.0 nM 7.75
CHEMBL2396952 IC50 = 36.0 nM 7.44
CHEMBL2396960 IC50 = 45.0 nM 7.35
CHEMBL2396963 IC50 = 49.0 nM 7.31
CHEMBL2396958 IC50 = 117.0 nM 6.93
CHEMBL2396965 IC50 = 127.0 nM 6.90
CHEMBL2396961 IC50 = 165.0 nM 6.78
CHEMBL2396966 IC50 = 190.0 nM 6.72
CHEMBL2396951 IC50 = 662.0 nM 6.18
CHEMBL2396967 IC50 = 2827.0 nM 5.55
CHEMBL2396962 IC50 = 6998.0 nM 5.16
CHEMBL2396952 IC50 = 30305.0 nM 4.52