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Assay Detail

CHEMBL2410098

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant His-tagged HDAC2 (1 to 488) (unknown origin) using MAL as substrate incubated for 3 hrs prior to substrate addition measured after 60 mins by fluorescence plate reader analysis
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 2 (CHEMBL1937)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent, isoform-selective inhibitors of histone deacetylase containing chiral heterocyclic capping groups and a N-(2-aminophenyl)benzamide binding unit.
Marson CM, Matthews CJ, Yiannaki E, Atkinson SJ, Soden PE, Shukla L, Lamadema N, Thomas NS.
J Med Chem (2013) 56 : 6156 -6174
PubMed 23829483 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 6.74 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL272980 MOCETINOSTAT 2.0 1 7.66
CHEMBL2407719 1 7.36
CHEMBL2407731 1 7.32
CHEMBL2407718 1 7.25
CHEMBL2407736 1 7.25
CHEMBL2407735 1 7.18
CHEMBL2407733 1 7.11
CHEMBL2407723 1 7.09
CHEMBL2407721 1 7.09
CHEMBL2407717 1 7.07
CHEMBL2407720 1 6.98
CHEMBL2407722 1 6.96
CHEMBL2407732 1 6.94
CHEMBL2407730 1 6.66
CHEMBL2407742 1 6.61
CHEMBL2407734 1 6.58
CHEMBL2407724 1 6.37
CHEMBL2407716 1 6.36
CHEMBL2407725 1 6.27
CHEMBL2407738 1 6.24
CHEMBL2407737 1 6.04
CHEMBL2407743 1 5.84
CHEMBL2407740 1 4.78
CHEMBL2407729 1 -
CHEMBL2407728 1 -
CHEMBL2407727 1 -
CHEMBL2407726 1 -
CHEMBL2407741 1 -
CHEMBL2407739 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL272980 MOCETINOSTAT IC50 = 22.0 nM 7.66
CHEMBL2407719 IC50 = 44.0 nM 7.36
CHEMBL2407731 IC50 = 48.0 nM 7.32
CHEMBL2407718 IC50 = 56.0 nM 7.25
CHEMBL2407736 IC50 = 56.0 nM 7.25
CHEMBL2407735 IC50 = 66.0 nM 7.18
CHEMBL2407733 IC50 = 78.0 nM 7.11
CHEMBL2407723 IC50 = 81.0 nM 7.09
CHEMBL2407721 IC50 = 82.0 nM 7.09
CHEMBL2407717 IC50 = 85.0 nM 7.07
CHEMBL2407720 IC50 = 104.0 nM 6.98
CHEMBL2407722 IC50 = 111.0 nM 6.96
CHEMBL2407732 IC50 = 116.0 nM 6.94
CHEMBL2407730 IC50 = 219.0 nM 6.66
CHEMBL2407742 IC50 = 248.0 nM 6.61
CHEMBL2407734 IC50 = 260.0 nM 6.58
CHEMBL2407724 IC50 = 425.0 nM 6.37
CHEMBL2407716 IC50 = 440.0 nM 6.36
CHEMBL2407725 IC50 = 538.0 nM 6.27
CHEMBL2407738 IC50 = 580.0 nM 6.24
CHEMBL2407737 IC50 = 920.0 nM 6.04
CHEMBL2407743 IC50 = 1430.0 nM 5.84
CHEMBL2407740 IC50 = 16700.0 nM 4.78
CHEMBL2407729 IC50 > 20000.0 nM -
CHEMBL2407728 IC50 > 20000.0 nM -
CHEMBL2407727 IC50 > 20000.0 nM -
CHEMBL2407726 IC50 > 20000.0 nM -
CHEMBL2407741 IC50 > 20000.0 nM -
CHEMBL2407739 IC50 > 20000.0 nM -