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Assay Detail

CHEMBL2412795

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]Epibatidine from rat alpha2beta4 nACHR by liquid scintillation counting analysis
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha2/beta4 (CHEMBL1907590)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Chemistry, pharmacology, and behavioral studies identify chiral cyclopropanes as selective α4β2-nicotinic acetylcholine receptor partial agonists exhibiting an antidepressant profile. Part II.
Zhang HK, Yu LF, Eaton JB, Whiteaker P, Onajole OK, Hanania T, Brunner D, Lukas RJ, Kozikowski AP.
J Med Chem (2013) 56 : 5495 -5504
PubMed 23734673 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 6.50 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2409637 1 7.70
CHEMBL2409631 1 7.29
CHEMBL3 NICOTINE 4.0 1 7.16
CHEMBL2409627 1 6.89
CHEMBL2409621 1 6.69
CHEMBL2409635 1 6.67
CHEMBL2409634 1 6.63
CHEMBL2409636 1 6.50
CHEMBL2409622 1 6.34
CHEMBL2409633 1 6.26
CHEMBL2409625 1 6.21
CHEMBL2409623 1 5.11
CHEMBL2409630 1 5.00
CHEMBL2409626 1 -
CHEMBL2409619 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2409637 Ki = 20.2 nM 7.70
CHEMBL2409631 Ki = 50.8 nM 7.29
CHEMBL3 NICOTINE Ki = 70.0 nM 7.16
CHEMBL2409627 Ki = 129.5 nM 6.89
CHEMBL2409621 Ki = 203.0 nM 6.69
CHEMBL2409635 Ki = 211.8 nM 6.67
CHEMBL2409634 Ki = 233.4 nM 6.63
CHEMBL2409636 Ki = 318.0 nM 6.50
CHEMBL2409622 Ki = 453.0 nM 6.34
CHEMBL2409633 Ki = 546.6 nM 6.26
CHEMBL2409625 Ki = 612.0 nM 6.21
CHEMBL2409623 Ki = 7850.0 nM 5.11
CHEMBL2409630 Ki = 9959.0 nM 5.00
CHEMBL2409626 Ki > 10000.0 nM -
CHEMBL2409619 Ki > 10000.0 nM -