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Assay Detail

CHEMBL2415549

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PARP-1 assessed as synthesis of [3H]-ADP-ribose polymers from [3H]-NAD+ by scintillation proximity assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Poly [ADP-ribose] polymerase 1 (CHEMBL3105)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

One-pot tandem Hurtley-retro-Claisen-cyclisation reactions in the synthesis of 3-substituted analogues of 5-aminoisoquinolin-1-one (5-AIQ), a water-soluble inhibitor of PARPs.
Woon EC, Sunderland PT, Paine HA, Lloyd MD, Thompson AS, Threadgill MD.
Bioorg Med Chem (2013) 21 : 5218 -5227
PubMed 23849206 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.04 6.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2414048 1 6.64
CHEMBL2414049 1 6.50
CHEMBL2414042 1 6.48
CHEMBL2414051 1 6.31
CHEMBL2414046 1 6.24
CHEMBL2414044 1 6.06
CHEMBL2414045 1 6.05
CHEMBL2414043 1 5.97
CHEMBL2414052 1 5.93
CHEMBL446240 1 5.80
CHEMBL2414050 1 5.29
CHEMBL2414047 1 5.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2414048 IC50 = 230.0 nM 6.64
CHEMBL2414049 IC50 = 320.0 nM 6.50
CHEMBL2414042 IC50 = 330.0 nM 6.48
CHEMBL2414051 IC50 = 490.0 nM 6.31
CHEMBL2414046 IC50 = 570.0 nM 6.24
CHEMBL2414044 IC50 = 880.0 nM 6.06
CHEMBL2414045 IC50 = 900.0 nM 6.05
CHEMBL2414043 IC50 = 1070.0 nM 5.97
CHEMBL2414052 IC50 = 1170.0 nM 5.93
CHEMBL446240 IC50 = 1600.0 nM 5.80
CHEMBL2414050 IC50 = 5140.0 nM 5.29
CHEMBL2414047 IC50 = 5610.0 nM 5.25