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Assay Detail

CHEMBL2421978

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of trypsin-activated human recombinant renin using (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(DABCYL)-Arg) as substrate incubated for 10 mins prior to substrate addition measured after 90 mins by fluorescence assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Renin (CHEMBL286)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and optimization of novel (3S,5R)-5-(2,2-dimethyl-5-oxo-4-phenylpiperazin-1-yl)piperidine-3-carboxamides as orally active renin inhibitors.
Mori Y, Ogawa Y, Mochizuki A, Nakamura Y, Fujimoto T, Sugita C, Miyazaki S, Tamaki K, Nagayama T, Nagai Y, Inoue S, Chiba K, Nishi T.
Bioorg Med Chem (2013) 21 : 5907 -5922
PubMed 23886807 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 8.72 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2419040 1 9.00
CHEMBL2419042 1 8.96
CHEMBL2419045 1 8.89
CHEMBL2204732 1 8.89
CHEMBL2419046 1 8.85
CHEMBL2419044 1 8.80
CHEMBL2204739 1 8.80
CHEMBL2419041 1 8.77
CHEMBL2419048 1 8.74
CHEMBL2419043 1 8.72
CHEMBL2204734 1 8.68
CHEMBL2204740 1 8.68
CHEMBL2204017 1 8.68
CHEMBL2419050 1 8.64
CHEMBL2204018 1 8.64
CHEMBL2419049 1 8.62
CHEMBL2204741 1 8.60
CHEMBL2419047 1 8.48
CHEMBL2419039 1 8.28

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2419040 IC50 = 1.0 nM 9.00
CHEMBL2419042 IC50 = 1.1 nM 8.96
CHEMBL2419045 IC50 = 1.3 nM 8.89
CHEMBL2204732 IC50 = 1.3 nM 8.89
CHEMBL2419046 IC50 = 1.4 nM 8.85
CHEMBL2419044 IC50 = 1.6 nM 8.80
CHEMBL2204739 IC50 = 1.6 nM 8.80
CHEMBL2419041 IC50 = 1.7 nM 8.77
CHEMBL2419048 IC50 = 1.8 nM 8.74
CHEMBL2419043 IC50 = 1.9 nM 8.72
CHEMBL2204734 IC50 = 2.1 nM 8.68
CHEMBL2204740 IC50 = 2.1 nM 8.68
CHEMBL2204017 IC50 = 2.1 nM 8.68
CHEMBL2419050 IC50 = 2.3 nM 8.64
CHEMBL2204018 IC50 = 2.3 nM 8.64
CHEMBL2419049 IC50 = 2.4 nM 8.62
CHEMBL2204741 IC50 = 2.5 nM 8.60
CHEMBL2419047 IC50 = 3.3 nM 8.48
CHEMBL2419039 IC50 = 5.2 nM 8.28