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Assay Detail

CHEMBL2422303

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant TRPC6 expressed in HEK293-MSRII cells assessed as carbachol-stimulated Ca2+/Na+ influx after 10 mins by FLIPR assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293-MSRII
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Short transient receptor potential channel 6 (CHEMBL2417347)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of potent blockers of the canonical transient receptor channels, TRPC3 and TRPC6, based on an anilino-thiazole pharmacophore.
Washburn DG, Holt DA, Dodson J, McAtee JJ, Terrell LR, Barton L, Manns S, Waszkiewicz A, Pritchard C, Gillie DJ, Morrow DM, Davenport EA, Lozinskaya IM, Guss J, Basilla JB, Negron LK, Klein M, Willette RN, Fries RE, Jensen TC, Xu X, Schnackenberg CG, Marino JP.
Bioorg Med Chem Lett (2013) 23 : 4979 -4984
PubMed 23886683 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.52 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2418809 1 8.40
CHEMBL2418807 1 7.80
CHEMBL2418811 1 7.80
CHEMBL2418808 1 7.60
CHEMBL2418815 1 7.50
CHEMBL2418810 1 7.30
CHEMBL2418814 1 7.20
CHEMBL2418806 1 7.10
CHEMBL2418823 1 6.80
CHEMBL2418813 1 6.80
CHEMBL2418818 1 6.40
CHEMBL1506485 1 6.30
CHEMBL2418824 1 6.20
CHEMBL2418822 1 6.20
CHEMBL2418820 1 6.10
CHEMBL2418819 1 6.10
CHEMBL2418816 1 5.90
CHEMBL2418812 1 5.80
CHEMBL2418804 1 5.80
CHEMBL2418821 1 5.50
CHEMBL2418805 1 5.40
CHEMBL2418817 1 5.20
CHEMBL2418825 1 4.80
CHEMBL2417565 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2418809 IC50 = 4.0 nM 8.40
CHEMBL2418807 IC50 = 16.0 nM 7.80
CHEMBL2418811 IC50 = 16.0 nM 7.80
CHEMBL2418808 IC50 = 25.0 nM 7.60
CHEMBL2418815 IC50 = 32.0 nM 7.50
CHEMBL2418810 IC50 = 50.0 nM 7.30
CHEMBL2418814 IC50 = 63.0 nM 7.20
CHEMBL2418806 IC50 = 80.0 nM 7.10
CHEMBL2418823 IC50 = 160.0 nM 6.80
CHEMBL2418813 IC50 = 160.0 nM 6.80
CHEMBL2418818 IC50 = 400.0 nM 6.40
CHEMBL1506485 IC50 = 500.0 nM 6.30
CHEMBL2418824 IC50 = 630.0 nM 6.20
CHEMBL2418822 IC50 = 630.0 nM 6.20
CHEMBL2418820 IC50 = 800.0 nM 6.10
CHEMBL2418819 IC50 = 800.0 nM 6.10
CHEMBL2418816 IC50 = 1260.0 nM 5.90
CHEMBL2418812 IC50 = 1600.0 nM 5.80
CHEMBL2418804 IC50 = 1600.0 nM 5.80
CHEMBL2418821 IC50 = 3160.0 nM 5.50
CHEMBL2418805 IC50 = 4000.0 nM 5.40
CHEMBL2418817 IC50 = 6300.0 nM 5.20
CHEMBL2418825 IC50 = 15800.0 nM 4.80
CHEMBL2417565 IC50 > 25000.0 nM -