Assay Detail
Binding
CHEMBL2433121
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human DAT expressed in MDCK cells using [3H]-dopamine as substrate after 10 mins by scintillation counting
5
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | MDCK |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Sodium-dependent dopamine transporter (CHEMBL238) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
The lignan (-)-hinokinin displays modulatory effects on human monoamine and GABA transporter activities.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.70 | 5.08 |
| Inhibition | 2 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL242011 | HINOKININ | — | 2 | 5.08 |
| CHEMBL399831 | — | — | 2 | 4.92 |
| CHEMBL2431797 | (-)-O-METHYLCUBEBIN | — | 1 | 4.11 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL242011 | HINOKININ | IC50 | = | 8350.0 | nM | 5.08 |
| CHEMBL399831 | — | IC50 | = | 12000.0 | nM | 4.92 |
| CHEMBL2431797 | (-)-O-METHYLCUBEBIN | IC50 | = | 78600.0 | nM | 4.11 |
| CHEMBL399831 | — | Inhibition | - | % | - | |
| CHEMBL242011 | HINOKININ | Inhibition | - | % | - |