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Assay Detail

CHEMBL2437894

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of FAM-Bid BH3 peptide from recombinant Mcl-1 (unknown origin) expressed in Escherichia coli BL21 after 10 mins by fluorescence polarization assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Design and application of a rigid quinazolone scaffold based on two-face Bim α-helix mimicking.
Zhang Z, Liang X, Li X, Song T, Chen Q, Sheng H.
Eur J Med Chem (2013) 69 : 711 -718
PubMed 24095762 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 5.96 6.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2436891 1 6.91
CHEMBL51483 GOSSYPOL 3.0 1 6.72
CHEMBL2436885 1 6.21
CHEMBL2436890 1 5.92
CHEMBL192032 1 5.92
CHEMBL2436886 1 5.70
CHEMBL2436887 1 5.55
CHEMBL2436888 1 5.49
CHEMBL2436889 1 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2436891 Ki = 123.0 nM 6.91
CHEMBL51483 GOSSYPOL Ki = 190.0 nM 6.72
CHEMBL2436885 Ki = 609.0 nM 6.21
CHEMBL2436890 Ki = 1214.0 nM 5.92
CHEMBL192032 Ki = 1211.0 nM 5.92
CHEMBL2436886 Ki = 1981.0 nM 5.70
CHEMBL2436887 Ki = 2816.0 nM 5.55
CHEMBL2436888 Ki = 3240.0 nM 5.49
CHEMBL2436889 Ki = 5981.0 nM 5.22