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Assay Detail

CHEMBL2446792

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant 11beta-HSD1 expressed in HEK293 cells assessed as inhibition of [1,2,-3H]-cortisone to [3H]cortisol preincubated for 10 mins before substrate addition by scintillation counting
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase 1 (CHEMBL4235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of sterically encumbered 11β-aminoprogesterone derivatives and evaluation as 11β-hydroxysteroid dehydrogenase inhibitors and mineralocorticoid receptor antagonists.
Pandya K, Dietrich D, Seibert J, Vederas JC, Odermatt A.
Bioorg Med Chem (2013) 21 : 6274 -6281
PubMed 24074876 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.11 6.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1563246 1 6.20
CHEMBL2440126 1 6.19
CHEMBL2440888 11BETA-HYDROXYPROGESTERONE 1 5.94
CHEMBL2440894 1 -
CHEMBL2440893 1 -
CHEMBL2440892 1 -
CHEMBL2440891 1 -
CHEMBL2440890 1 -
CHEMBL2440889 11BETA-AMINOPROGESTERONE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1563246 IC50 = 630.0 nM 6.20
CHEMBL2440126 IC50 = 640.0 nM 6.19
CHEMBL2440888 11BETA-HYDROXYPROGESTERONE IC50 = 1160.0 nM 5.94
CHEMBL2440894 IC50 - -
CHEMBL2440893 IC50 - -
CHEMBL2440892 IC50 - -
CHEMBL2440891 IC50 - -
CHEMBL2440890 IC50 - -
CHEMBL2440889 11BETA-AMINOPROGESTERONE IC50 - -