Assay Detail
Binding
CHEMBL3049465
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as reduction in initial velocity at the step of threonine activation preincubated for 10 min with 4 uM enzyme before threonine addition measured after 30 min by HPLC analysis
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Phytophthora sojae |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Borrelidin, a potent antifungal agent: insight into the antifungal mechanism against Phytophthora sojae.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 1 | 5.00 | 5.00 |
| IC50 | 1 | 5.10 | 5.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL70590 | BORRELIDIN | — | 2 | 5.10 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL70590 | BORRELIDIN | IC50 | = | 8000.0 | nM | 5.10 |
| CHEMBL70590 | BORRELIDIN | Ki | = | 10000.0 | nM | 5.00 |