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Assay Detail

CHEMBL3049709

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Binding
Inhibition of PDHc in Pisum sativum Zhongwan-2 (pea) shoot mitochondria assessed as decrease in rate of NADH formation using sodium pyruvate as substrate preincubated for 25 min before substrate addition measured after 1 min by spectrophotometric assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pisum sativum
Tissue Shoot
Subcellular Mitochondria
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Pyruvate dehydrogenase complex (CHEMBL2366571)
Type PROTEIN COMPLEX
Organism Pisum sativum

Publication

Studies of O,O-dimethyl α-(2,4-dichlorophenoxyacetoxy)ethylphosphonate (HW02) as a new herbicide. 1. Synthesis and herbicidal activity of HW02 and analogues as novel inhibitors of pyruvate dehydrogenase complex.
He HW, Yuan JL, Peng H, Chen T, Shen P, Wan SQ, Li Y, Tan HL, He YH, He JB, Li Y.
J Agric Food Chem (2011) 59 : 4801 -4813
PubMed 21452831 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.74 4.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2227911 CLACYFOS 1 4.74
CHEMBL2227929 1 -
CHEMBL367623 2,4-DICHLOROPHENOXYACETIC ACID 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2227911 CLACYFOS IC50 = 18190.0 nM 4.74
CHEMBL2227929 IC50 = 442000.0 nM -
CHEMBL367623 2,4-DICHLOROPHENOXYACETIC ACID IC50 > 4530000.0 nM -