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Assay Detail

CHEMBL3072144

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of cathepsin D (unknown origin) using hemoglobin as substrate after 30 min by spectrophotometric analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cathepsin D (CHEMBL2581)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhibition of Cathepsin D protease activity by Punica granatum fruit peel extracts, isolates, and semisynthetic analogs
Chaturvedi AK, Luqman S, Dubey V, Thakur JP, Saikia D, Chanotiya CS, Shanker K, Negi AS
Med Chem Res (2013) 22 : 3953 -3958
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.71 5.03

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL221543 BETA-SITOSTENONE 1 5.03
CHEMBL169 URSOLIC ACID 2.0 1 4.74
CHEMBL43185 PIPERINE 2.0 1 4.37
CHEMBL487887 URSONIC ACID 1 -
CHEMBL260673 METHYL URSOLATE 1 -
CHEMBL296588 PEPSTATIN 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL221543 BETA-SITOSTENONE IC50 = 9400.0 nM 5.03
CHEMBL169 URSOLIC ACID IC50 = 18200.0 nM 4.74
CHEMBL43185 PIPERINE IC50 = 42300.0 nM 4.37
CHEMBL487887 URSONIC ACID IC50 = 6.41 ug.mL-1 -
CHEMBL260673 METHYL URSOLATE IC50 = 4.45 ug.mL-1 -
CHEMBL296588 PEPSTATIN IC50 = 0.0016 ug.mL-1 -