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Assay Detail

CHEMBL3082114

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of carboxylesterase in Fischer 344 Rattus norvegicus (rat) liver microsomes using p-nitrophenyl acetate as substrate incubated for 5 min prior to substrate addition measured for 2 min by spectrophotometric analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Liver carboxylesterase B-1 (CHEMBL2366498)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis of new carboxylesterase inhibitors and evaluation of potency and water solubility.
Wheelock CE, Severson TF, Hammock BD.
Chem Res Toxicol (2001) 14 : 1563 -1572
PubMed 11743738 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 7.07 8.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL467451 1 8.20
CHEMBL467450 1 7.99
CHEMBL460807 1 7.61
CHEMBL89506 1 7.43
CHEMBL440542 1 7.26
CHEMBL270374 1 6.94
CHEMBL460809 1 6.79
CHEMBL270375 1 6.79
CHEMBL449775 1 5.89
CHEMBL2268509 1 5.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL467451 IC50 = 6.3 nM 8.20
CHEMBL467450 IC50 = 10.3 nM 7.99
CHEMBL460807 IC50 = 24.5 nM 7.61
CHEMBL89506 IC50 = 37.2 nM 7.43
CHEMBL440542 IC50 = 54.6 nM 7.26
CHEMBL270374 IC50 = 115.0 nM 6.94
CHEMBL460809 IC50 = 162.0 nM 6.79
CHEMBL270375 IC50 = 164.0 nM 6.79
CHEMBL449775 IC50 = 1290.0 nM 5.89
CHEMBL2268509 IC50 = 1500.0 nM 5.82