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Assay Detail

CHEMBL3089002

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Growth inhibition of Chinese hamster R2 cells expressing human PCFT4 after 96 hrs by CellTiter-blue assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cricetulus griseus
Cell Type R2
Confidence 1 — Organism
Curated By Autocuration

Target

R2 (CHEMBL614385)
Type CELL-LINE
Organism Homo sapiens

Publication

Tumor-targeting with novel non-benzoyl 6-substituted straight chain pyrrolo[2,3-d]pyrimidine antifolates via cellular uptake by folate receptor α and inhibition of de novo purine nucleotide biosynthesis.
Wang Y, Cherian C, Orr S, Mitchell-Ryan S, Hou Z, Raghavan S, Matherly LH, Gangjee A.
J Med Chem (2013) 56 : 8684 -8695
PubMed 24111942 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.25 7.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225072 PEMETREXED 4.0 1 7.88
CHEMBL192632 1 7.64
CHEMBL34412 LOMETREXOL 2.0 1 7.42
CHEMBL1201746 PRALATREXATE 4.0 1 7.24
CHEMBL225071 RALTITREXED 4.0 1 7.00
CHEMBL34259 METHOTREXATE 4.0 1 6.92
CHEMBL365307 1 6.67
CHEMBL502528 1 -
CHEMBL3086867 1 -
CHEMBL3086866 1 -
CHEMBL3086865 1 -
CHEMBL3086864 1 -
CHEMBL3086863 1 -
CHEMBL3086673 1 -
CHEMBL3086868 1 -
CHEMBL526928 1 -
CHEMBL3086869 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225072 PEMETREXED IC50 = 13.2 nM 7.88
CHEMBL192632 IC50 = 23.0 nM 7.64
CHEMBL34412 LOMETREXOL IC50 = 38.0 nM 7.42
CHEMBL1201746 PRALATREXATE IC50 = 57.0 nM 7.24
CHEMBL225071 RALTITREXED IC50 = 99.5 nM 7.00
CHEMBL34259 METHOTREXATE IC50 = 121.0 nM 6.92
CHEMBL365307 IC50 = 213.0 nM 6.67
CHEMBL502528 IC50 > 1000.0 nM -
CHEMBL3086867 IC50 > 1000.0 nM -
CHEMBL3086866 IC50 > 1000.0 nM -
CHEMBL3086865 IC50 > 1000.0 nM -
CHEMBL3086864 IC50 > 1000.0 nM -
CHEMBL3086863 IC50 > 1000.0 nM -
CHEMBL3086673 IC50 > 1000.0 nM -
CHEMBL3086868 IC50 > 1000.0 nM -
CHEMBL526928 IC50 > 1000.0 nM -
CHEMBL3086869 IC50 > 1000.0 nM -