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Assay Detail

CHEMBL3089095

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human IDO1 expressed in HEK293 cells assessed as kynurenine release after 5 hrs by spectrophotometry
15
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Indoleamine 2,3-dioxygenase 1 (CHEMBL4685)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of tryptanthrin derivatives as potent inhibitors of indoleamine 2,3-dioxygenase with therapeutic activity in Lewis lung cancer (LLC) tumor-bearing mice.
Yang S, Li X, Hu F, Li Y, Yang Y, Yan J, Kuang C, Yang Q.
J Med Chem (2013) 56 : 8321 -8331
PubMed 24099220 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.16 10.74
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL432537 1 10.74
CHEMBL1098875 1 7.64
CHEMBL3087012 1 7.61
CHEMBL3087010 1 7.57
CHEMBL306946 TRYPTANTHRIN 1 7.27
CHEMBL72165 1 7.27
CHEMBL1276265 1 6.74
CHEMBL3087014 1 6.73
CHEMBL3087009 1 6.33
CHEMBL2314972 1 6.11
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN 1 4.74
CHEMBL312537 1 -
CHEMBL3086870 1 -
CHEMBL3087011 1 -
CHEMBL3087013 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL432537 IC50 = 0.018 nM 10.74
CHEMBL1098875 IC50 = 23.0 nM 7.64
CHEMBL3087012 IC50 = 24.3 nM 7.61
CHEMBL3087010 IC50 = 27.1 nM 7.57
CHEMBL306946 TRYPTANTHRIN IC50 = 53.7 nM 7.27
CHEMBL72165 IC50 = 53.9 nM 7.27
CHEMBL1276265 IC50 = 183.0 nM 6.74
CHEMBL3087014 IC50 = 185.0 nM 6.73
CHEMBL3087009 IC50 = 466.0 nM 6.33
CHEMBL2314972 IC50 = 779.0 nM 6.11
CHEMBL504816 (L)-1-METHYLTRYPTOPHAN IC50 = 18400.0 nM 4.74
CHEMBL312537 IC50 - -
CHEMBL3086870 IC50 - -
CHEMBL3087011 IC50 - -
CHEMBL3087013 Inhibition - % -