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Assay Detail

CHEMBL3096167

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 6xHis-tagged recombinant FAP (unknown origin) expressed in baculovirus expression system assessed as hydrolysis of Nle-Pro-aminomethylcoumarin after 20 mins by fluorometric assay
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prolyl endopeptidase FAP (CHEMBL4683)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of pyrrolidine-2-carbonitrile and 4-fluoropyrrolidine-2-carbonitrile derivatives as dipeptidyl peptidase-4 inhibitors for the treatment of type 2 diabetes.
Wang J, Feng Y, Ji X, Deng G, Leng Y, Liu H.
Bioorg Med Chem (2013) 21 : 7418 -7429
PubMed 24153396 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 5.90 7.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3093903 1 7.09
CHEMBL3093905 1 6.85
CHEMBL3093910 1 6.85
CHEMBL3093913 1 6.82
CHEMBL3091488 1 6.66
CHEMBL3093914 1 6.46
CHEMBL3093904 1 6.33
CHEMBL3093907 1 6.33
CHEMBL3093908 1 6.21
CHEMBL3093923 1 6.10
CHEMBL3093915 1 6.05
CHEMBL200347 1 5.85
CHEMBL3093919 1 5.84
CHEMBL3093902 1 5.82
CHEMBL3093918 1 5.80
CHEMBL3093900 1 5.80
CHEMBL3093909 1 5.77
CHEMBL3093920 1 5.67
CHEMBL3093916 1 5.65
CHEMBL3093899 1 5.58
CHEMBL3093896 1 5.57
CHEMBL3093925 1 5.52
CHEMBL3093906 1 5.44
CHEMBL142703 VILDAGLIPTIN 4.0 1 5.43
CHEMBL3093911 1 5.41
CHEMBL3093897 1 5.24
CHEMBL3093912 1 5.21
CHEMBL3093924 1 5.10
CHEMBL3093898 1 4.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3093903 IC50 = 82.0 nM 7.09
CHEMBL3093905 IC50 = 140.0 nM 6.85
CHEMBL3093910 IC50 = 140.0 nM 6.85
CHEMBL3093913 IC50 = 150.0 nM 6.82
CHEMBL3091488 IC50 = 220.0 nM 6.66
CHEMBL3093914 IC50 = 350.0 nM 6.46
CHEMBL3093904 IC50 = 470.0 nM 6.33
CHEMBL3093907 IC50 = 470.0 nM 6.33
CHEMBL3093908 IC50 = 610.0 nM 6.21
CHEMBL3093923 IC50 = 800.0 nM 6.10
CHEMBL3093915 IC50 = 900.0 nM 6.05
CHEMBL200347 IC50 = 1410.0 nM 5.85
CHEMBL3093919 IC50 = 1460.0 nM 5.84
CHEMBL3093902 IC50 = 1530.0 nM 5.82
CHEMBL3093918 IC50 = 1580.0 nM 5.80
CHEMBL3093900 IC50 = 1580.0 nM 5.80
CHEMBL3093909 IC50 = 1700.0 nM 5.77
CHEMBL3093920 IC50 = 2140.0 nM 5.67
CHEMBL3093916 IC50 = 2220.0 nM 5.65
CHEMBL3093899 IC50 = 2600.0 nM 5.58
CHEMBL3093896 IC50 = 2690.0 nM 5.57
CHEMBL3093925 IC50 = 3010.0 nM 5.52
CHEMBL3093906 IC50 = 3650.0 nM 5.44
CHEMBL142703 VILDAGLIPTIN IC50 = 3720.0 nM 5.43
CHEMBL3093911 IC50 = 3910.0 nM 5.41
CHEMBL3093897 IC50 = 5810.0 nM 5.24
CHEMBL3093912 IC50 = 6100.0 nM 5.21
CHEMBL3093924 IC50 = 7870.0 nM 5.10
CHEMBL3093898 IC50 = 27630.0 nM 4.56