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Assay Detail

CHEMBL3096448

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mPGES-1 from human A549 cell microsomal membranes using PGH2 as substrate incubated 15 mins prior to substrate addition measured after 1 min by RP-HPLC analysis
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A549
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E synthase (CHEMBL5658)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Aminothiazole-featured pirinixic acid derivatives as dual 5-lipoxygenase and microsomal prostaglandin E2 synthase-1 inhibitors with improved potency and efficiency in vivo.
Hanke T, Dehm F, Liening S, Popella SD, Maczewsky J, Pillong M, Kunze J, Weinigel C, Barz D, Kaiser A, Wurglics M, Lämmerhofer M, Schneider G, Sautebin L, Schubert-Zsilavecz M, Werz O.
J Med Chem (2013) 56 : 9031 -9044
PubMed 24171493 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.03 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3094419 1 6.52
CHEMBL3094412 1 6.40
CHEMBL3094418 1 6.40
CHEMBL3094420 1 6.40
CHEMBL3094421 1 6.40
CHEMBL3091497 1 6.30
CHEMBL3094422 1 6.30
CHEMBL3094428 1 6.22
CHEMBL3094414 1 6.22
CHEMBL3094413 1 6.22
CHEMBL3094425 1 6.16
CHEMBL3094431 1 6.16
CHEMBL3091499 1 6.16
CHEMBL3091498 1 6.10
CHEMBL3091500 1 6.10
CHEMBL3094424 1 6.05
CHEMBL3094432 1 5.92
CHEMBL3094423 1 5.89
CHEMBL3094426 1 5.89
CHEMBL3094433 1 5.85
CHEMBL3091495 1 5.85
CHEMBL3094415 1 5.80
CHEMBL3094411 1 5.77
CHEMBL3094427 1 5.77
CHEMBL3094416 1 5.75
CHEMBL3094410 1 5.72
CHEMBL3091496 1 5.64
CHEMBL29097 1 5.62
CHEMBL3094417 1 5.30
CHEMBL93 ZILEUTON 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3094419 IC50 = 300.0 nM 6.52
CHEMBL3094412 IC50 = 400.0 nM 6.40
CHEMBL3094418 IC50 = 400.0 nM 6.40
CHEMBL3094420 IC50 = 400.0 nM 6.40
CHEMBL3094421 IC50 = 400.0 nM 6.40
CHEMBL3091497 IC50 = 500.0 nM 6.30
CHEMBL3094422 IC50 = 500.0 nM 6.30
CHEMBL3094428 IC50 = 600.0 nM 6.22
CHEMBL3094414 IC50 = 600.0 nM 6.22
CHEMBL3094413 IC50 = 600.0 nM 6.22
CHEMBL3094425 IC50 = 700.0 nM 6.16
CHEMBL3094431 IC50 = 700.0 nM 6.16
CHEMBL3091499 IC50 = 700.0 nM 6.16
CHEMBL3091498 IC50 = 800.0 nM 6.10
CHEMBL3091500 IC50 = 800.0 nM 6.10
CHEMBL3094424 IC50 = 900.0 nM 6.05
CHEMBL3094432 IC50 = 1200.0 nM 5.92
CHEMBL3094423 IC50 = 1300.0 nM 5.89
CHEMBL3094426 IC50 = 1300.0 nM 5.89
CHEMBL3094433 IC50 = 1400.0 nM 5.85
CHEMBL3091495 IC50 = 1400.0 nM 5.85
CHEMBL3094415 IC50 = 1600.0 nM 5.80
CHEMBL3094411 IC50 = 1700.0 nM 5.77
CHEMBL3094427 IC50 = 1700.0 nM 5.77
CHEMBL3094416 IC50 = 1800.0 nM 5.75
CHEMBL3094410 IC50 = 1900.0 nM 5.72
CHEMBL3091496 IC50 = 2300.0 nM 5.64
CHEMBL29097 IC50 = 2400.0 nM 5.62
CHEMBL3094417 IC50 = 5000.0 nM 5.30
CHEMBL93 ZILEUTON IC50 - -