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Assay Detail

CHEMBL3096511

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of ovalbumin-induced antigen presentation in mouse A20 cells treated 1 hr before ovalbumin addition incubated for 16 hrs followed by co-cultured with DO10.10 T-cell hybridoma cells for 24 hrs assessed as IL-2 production by luminex single-bead analysis
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type A20
Confidence 1 — Organism
Curated By Autocuration

Target

A20 (CHEMBL613850)
Type CELL-LINE
Organism Mus musculus

Publication

Identification of potent and selective cathepsin S inhibitors containing different central cyclic scaffolds.
Hilpert H, Mauser H, Humm R, Anselm L, Kuehne H, Hartmann G, Gruener S, Banner DW, Benz J, Gsell B, Kuglstatter A, Stihle M, Thoma R, Sanchez RA, Iding H, Wirz B, Haap W.
J Med Chem (2013) 56 : 9789 -9801
PubMed 24224654 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 7.21 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3093822 1 9.00
CHEMBL3093935 1 8.70
CHEMBL3093942 1 8.70
CHEMBL3093826 1 8.70
CHEMBL3093941 1 8.52
CHEMBL3093825 1 8.30
CHEMBL3093947 1 7.92
CHEMBL3093934 1 7.82
CHEMBL3093823 1 7.80
CHEMBL3093827 1 7.77
CHEMBL3093936 1 7.72
CHEMBL3093940 1 7.64
CHEMBL3093928 1 7.54
CHEMBL3093946 1 7.37
CHEMBL3093929 1 7.36
CHEMBL3093932 1 7.24
CHEMBL3093931 1 7.04
CHEMBL3093945 1 6.93
CHEMBL3093944 1 6.68
CHEMBL3093933 1 6.66
CHEMBL3093948 1 6.63
CHEMBL3093824 1 6.61
CHEMBL3093829 1 6.43
CHEMBL3093927 1 6.41
CHEMBL3093930 1 6.24
CHEMBL3093926 1 6.09
CHEMBL3093938 1 6.03
CHEMBL3093828 1 5.87
CHEMBL3093943 1 5.37
CHEMBL3093939 1 5.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3093822 IC50 = 1.0 nM 9.00
CHEMBL3093935 IC50 = 2.0 nM 8.70
CHEMBL3093942 IC50 = 2.0 nM 8.70
CHEMBL3093826 IC50 = 2.0 nM 8.70
CHEMBL3093941 IC50 = 3.0 nM 8.52
CHEMBL3093825 IC50 = 5.0 nM 8.30
CHEMBL3093947 IC50 = 12.0 nM 7.92
CHEMBL3093934 IC50 = 15.0 nM 7.82
CHEMBL3093823 IC50 = 16.0 nM 7.80
CHEMBL3093827 IC50 = 17.0 nM 7.77
CHEMBL3093936 IC50 = 19.0 nM 7.72
CHEMBL3093940 IC50 = 23.0 nM 7.64
CHEMBL3093928 IC50 = 29.0 nM 7.54
CHEMBL3093946 IC50 = 43.0 nM 7.37
CHEMBL3093929 IC50 = 44.0 nM 7.36
CHEMBL3093932 IC50 = 58.0 nM 7.24
CHEMBL3093931 IC50 = 91.0 nM 7.04
CHEMBL3093945 IC50 = 118.0 nM 6.93
CHEMBL3093944 IC50 = 208.0 nM 6.68
CHEMBL3093933 IC50 = 217.0 nM 6.66
CHEMBL3093948 IC50 = 236.0 nM 6.63
CHEMBL3093824 IC50 = 248.0 nM 6.61
CHEMBL3093829 IC50 = 371.0 nM 6.43
CHEMBL3093927 IC50 = 391.0 nM 6.41
CHEMBL3093930 IC50 = 572.0 nM 6.24
CHEMBL3093926 IC50 = 816.0 nM 6.09
CHEMBL3093938 IC50 = 925.0 nM 6.03
CHEMBL3093828 IC50 = 1340.0 nM 5.87
CHEMBL3093943 IC50 = 4239.0 nM 5.37
CHEMBL3093939 IC50 = 4506.0 nM 5.35