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Assay Detail

CHEMBL3096841

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant human P2X3 receptor expressed in Xenopus oocytes assessed as inhibition of ATP-induced ion current preincubated for 20 mins followed by ATP addition by two-electrode voltage clamp assay
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2X purinoceptor 3 (CHEMBL2998)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and synthesis of potent and selective P2X₃ receptor antagonists derived from PPADS as potential pain modulators.
Cho JH, Jung KY, Jung Y, Kim MH, Ko H, Park CS, Kim YC.
Eur J Med Chem (2013) 70 : 811 -830
PubMed 24246730 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 6.25 7.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL596234 A-317491 1 7.46
CHEMBL3091630 1 7.22
CHEMBL3091628 1 6.84
CHEMBL3091631 1 6.44
CHEMBL3091632 1 6.42
CHEMBL3091610 1 6.34
CHEMBL3091607 1 6.34
CHEMBL3091619 1 6.32
CHEMBL3091620 1 6.23
CHEMBL3091629 1 6.20
CHEMBL3091633 1 6.20
CHEMBL3091635 1 6.19
CHEMBL3091625 1 6.14
CHEMBL3091605 1 6.10
CHEMBL3091618 1 6.08
CHEMBL3091634 1 6.07
CHEMBL3091606 1 6.04
CHEMBL3091627 1 6.03
CHEMBL3091622 1 5.98
CHEMBL3091617 1 5.90
CHEMBL3091623 1 5.53
CHEMBL3091621 1 5.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL596234 A-317491 IC50 = 35.0 nM 7.46
CHEMBL3091630 IC50 = 60.0 nM 7.22
CHEMBL3091628 IC50 = 145.0 nM 6.84
CHEMBL3091631 IC50 = 366.0 nM 6.44
CHEMBL3091632 IC50 = 377.0 nM 6.42
CHEMBL3091610 IC50 = 458.0 nM 6.34
CHEMBL3091607 IC50 = 460.0 nM 6.34
CHEMBL3091619 IC50 = 474.0 nM 6.32
CHEMBL3091620 IC50 = 587.0 nM 6.23
CHEMBL3091629 IC50 = 636.0 nM 6.20
CHEMBL3091633 IC50 = 638.0 nM 6.20
CHEMBL3091635 IC50 = 652.0 nM 6.19
CHEMBL3091625 IC50 = 723.0 nM 6.14
CHEMBL3091605 IC50 = 797.0 nM 6.10
CHEMBL3091618 IC50 = 824.0 nM 6.08
CHEMBL3091634 IC50 = 856.0 nM 6.07
CHEMBL3091606 IC50 = 920.0 nM 6.04
CHEMBL3091627 IC50 = 928.0 nM 6.03
CHEMBL3091622 IC50 = 1050.0 nM 5.98
CHEMBL3091617 IC50 = 1250.0 nM 5.90
CHEMBL3091623 IC50 = 2930.0 nM 5.53
CHEMBL3091621 IC50 = 3300.0 nM 5.48