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Assay Detail

CHEMBL3096860

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NAMPT in human HepG2 cells using [14C]-nicotinamide/PRPP as substrate assessed as formation of [14C]-nicotinamide mononucleotide after 1 hr by liquid scintillation counting analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nicotinamide phosphoribosyltransferase (CHEMBL1744525)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Nicotinamide phosphoribosyltransferase inhibitors, design, preparation, and structure-activity relationship.
Christensen MK, Erichsen KD, Olesen UH, Tjørnelund J, Fristrup P, Thougaard A, Nielsen SJ, Sehested M, Jensen PB, Loza E, Kalvinsh I, Garten A, Kiess W, Björkling F.
J Med Chem (2013) 56 : 9071 -9088
PubMed 24164086 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.22 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3094250 1 9.70
CHEMBL3094255 1 9.52
CHEMBL3094249 1 8.96
CHEMBL566757 DAPORINAD 2.0 1 8.66
CHEMBL3094254 1 8.62
CHEMBL3094258 1 8.62
CHEMBL3094257 1 8.49
CHEMBL3094229 1 8.44
CHEMBL3094251 1 8.36
CHEMBL3094236 1 8.09
CHEMBL3094238 1 7.93
CHEMBL17289 CHS-828 1.0 1 7.74
CHEMBL3094224 1 7.64
CHEMBL2417795 1 7.42
CHEMBL3094234 1 7.31
CHEMBL3094230 1 7.30
CHEMBL3094237 1 6.88

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3094250 IC50 = 0.2 nM 9.70
CHEMBL3094255 IC50 = 0.3 nM 9.52
CHEMBL3094249 IC50 = 1.1 nM 8.96
CHEMBL566757 DAPORINAD IC50 = 2.2 nM 8.66
CHEMBL3094254 IC50 = 2.4 nM 8.62
CHEMBL3094258 IC50 = 2.4 nM 8.62
CHEMBL3094257 IC50 = 3.2 nM 8.49
CHEMBL3094229 IC50 = 3.6 nM 8.44
CHEMBL3094251 IC50 = 4.4 nM 8.36
CHEMBL3094236 IC50 = 8.2 nM 8.09
CHEMBL3094238 IC50 = 11.8 nM 7.93
CHEMBL17289 CHS-828 IC50 = 18.3 nM 7.74
CHEMBL3094224 IC50 = 22.8 nM 7.64
CHEMBL2417795 IC50 = 38.5 nM 7.42
CHEMBL3094234 IC50 = 49.0 nM 7.31
CHEMBL3094230 IC50 = 49.6 nM 7.30
CHEMBL3094237 IC50 = 133.2 nM 6.88