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Assay Detail

CHEMBL3101220

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TNF-alpha-induced NF-kappaB transcriptional activation in human HepG2 cells preincubated for 1 hr followed by TNF-alpha challenge measured after 1 hr by luciferase reporter gene assay
7
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

New anti-inflammatory cembranoid diterpenoids from the Vietnamese soft coral Lobophytum crassum.
Thao NP, Luyen BT, Ngan NT, Song SB, Cuong NX, Nam NH, Kiem PV, Kim YH, Minh CV.
Bioorg Med Chem Lett (2014) 24 : 228 -232
PubMed 24314396 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.48 6.05
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL421 SULFASALAZINE 4.0 1 6.05
CHEMBL3099358 2 5.20
CHEMBL3099355 2 5.18
CHEMBL3099357 1 -
CHEMBL3099356 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL421 SULFASALAZINE IC50 = 900.0 nM 6.05
CHEMBL3099358 IC50 = 6300.0 nM 5.20
CHEMBL3099355 IC50 = 6630.0 nM 5.18
CHEMBL3099355 Inhibition - % -
CHEMBL3099358 Inhibition - % -
CHEMBL3099357 IC50 > 20000.0 nM -
CHEMBL3099356 IC50 > 20000.0 nM -