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Assay Detail

CHEMBL3106280

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at rat CB1 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 30 mins
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cannabinoid receptor 1 (CHEMBL3571)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Controlled-deactivation cannabinergic ligands.
Sharma R, Nikas SP, Paronis CA, Wood JT, Halikhedkar A, Guo JJ, Thakur GA, Kulkarni S, Benchama O, Raghav JG, Gifford RS, Järbe TU, Bergman J, Makriyannis A.
J Med Chem (2013) 56 : 10142 -10157
PubMed 24286207 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 9.03 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3104362 1 9.40
CHEMBL3104360 1 9.30
CHEMBL3104358 1 8.38

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3104362 EC50 = 0.4 nM 9.40
CHEMBL3104360 EC50 = 0.5 nM 9.30
CHEMBL3104358 EC50 = 4.2 nM 8.38