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Assay Detail

CHEMBL3106549

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human N-terminal polyHis-tagged GRP94 (L69 to N337) expressed in Escherichia coli BL21(DE3) after 3 hrs by fluorescence polarization assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Endoplasmin (CHEMBL1075323)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Correlation between chemotype-dependent binding conformations of HSP90α/β and isoform selectivity-Implications for the structure-based design of HSP90α/β selective inhibitors for treating neurodegenerative diseases.
Ernst JT, Liu M, Zuccola H, Neubert T, Beaumont K, Turnbull A, Kallel A, Vought B, Stamos D.
Bioorg Med Chem Lett (2014) 24 : 204 -208
PubMed 24332488 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.96 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL252164 LUMINESPIB 2.0 1 8.40
CHEMBL3104859 1 8.30
CHEMBL467399 BIIB021 2.0 1 6.75
CHEMBL3104858 1 6.43
CHEMBL560895 SNX-2112 -1.0 1 6.32
CHEMBL3104860 1 5.58

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL252164 LUMINESPIB Ki = 4.0 nM 8.40
CHEMBL3104859 Ki = 5.0 nM 8.30
CHEMBL467399 BIIB021 Ki = 176.0 nM 6.75
CHEMBL3104858 Ki = 375.0 nM 6.43
CHEMBL560895 SNX-2112 Ki = 484.0 nM 6.32
CHEMBL3104860 Ki = 2650.0 nM 5.58