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Assay Detail

CHEMBL3107684

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [33P]-2MeS-ADP from human P2Y1 receptor expressed in HEK293 cells after 1 hr by scintillation counting analysis
36
Total Activities
36
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2Y purinoceptor 1 (CHEMBL4315)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Conformationally constrained ortho-anilino diaryl ureas: discovery of 1-(2-(1'-neopentylspiro[indoline-3,4'-piperidine]-1-yl)phenyl)-3-(4-(trifluoromethoxy)phenyl)urea, a potent, selective, and bioavailable P2Y1 antagonist.
Qiao JX, Wang TC, Ruel R, Thibeault C, L'Heureux A, Schumacher WA, Spronk SA, Hiebert S, Bouthillier G, Lloyd J, Pi Z, Schnur DM, Abell LM, Hua J, Price LA, Liu E, Wu Q, Steinbacher TE, Bostwick JS, Chang M, Zheng J, Gao Q, Ma B, McDonnell PA, Huang CS, Rehfuss R, Wexler RR, Lam PY.
J Med Chem (2013) 56 : 9275 -9295
PubMed 24164581 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 34 7.29 8.37
IC50 2 6.88 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3104636 1 8.37
CHEMBL3105199 1 8.34
CHEMBL3105201 1 8.24
CHEMBL3104624 1 8.23
CHEMBL2333770 1 8.22
CHEMBL3103636 1 8.18
CHEMBL3102866 1 8.14
CHEMBL3103635 1 8.12
CHEMBL3103625 1 8.06
CHEMBL3105195 1 7.94
CHEMBL3105200 1 7.89
CHEMBL3104630 1 7.88
CHEMBL3103627 1 7.80
CHEMBL3104635 1 7.73
CHEMBL3103626 1 7.69
CHEMBL3104634 1 7.68
CHEMBL3103634 1 7.58
CHEMBL3105196 1 7.54
CHEMBL3103630 1 7.53
CHEMBL3103629 1 7.44
CHEMBL3104625 1 7.44
CHEMBL3103632 1 7.41
CHEMBL3103631 1 7.19
CHEMBL3103624 1 7.12
CHEMBL3103633 1 7.03
CHEMBL3104631 1 6.70
CHEMBL3103623 1 6.64
CHEMBL3105198 1 6.60
CHEMBL3104633 1 6.52
CHEMBL3104629 1 6.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3104636 Ki = 4.3 nM 8.37
CHEMBL3105199 Ki = 4.6 nM 8.34
CHEMBL3105201 Ki = 5.8 nM 8.24
CHEMBL3104624 Ki = 5.9 nM 8.23
CHEMBL2333770 Ki = 6.0 nM 8.22
CHEMBL3103636 Ki = 6.6 nM 8.18
CHEMBL3102866 Ki = 7.3 nM 8.14
CHEMBL3103635 Ki = 7.6 nM 8.12
CHEMBL3103625 Ki = 8.7 nM 8.06
CHEMBL3105195 Ki = 11.5 nM 7.94
CHEMBL3105200 Ki = 12.8 nM 7.89
CHEMBL3104630 Ki = 13.3 nM 7.88
CHEMBL3103627 Ki = 16.0 nM 7.80
CHEMBL3104635 Ki = 18.6 nM 7.73
CHEMBL3103626 Ki = 20.3 nM 7.69
CHEMBL3104634 Ki = 20.7 nM 7.68
CHEMBL3103634 Ki = 26.0 nM 7.58
CHEMBL3105196 Ki = 28.7 nM 7.54
CHEMBL3103630 Ki = 29.5 nM 7.53
CHEMBL3104625 Ki = 36.6 nM 7.44
CHEMBL3103629 Ki = 36.5 nM 7.44
CHEMBL3103632 Ki = 39.0 nM 7.41
CHEMBL3103631 Ki = 65.0 nM 7.19
CHEMBL3103624 IC50 = 75.0 nM 7.12
CHEMBL3103633 Ki = 94.0 nM 7.03
CHEMBL3104631 Ki = 200.0 nM 6.70
CHEMBL3103623 IC50 = 227.0 nM 6.64
CHEMBL3105198 Ki = 253.0 nM 6.60
CHEMBL3104633 Ki = 300.0 nM 6.52
CHEMBL3104629 Ki = 390.0 nM 6.41
CHEMBL3105197 Ki = 617.2 nM 6.21
CHEMBL3104632 Ki = 690.0 nM 6.16
CHEMBL3103628 Ki = 830.0 nM 6.08
CHEMBL3104626 Ki = 4500.0 nM 5.35
CHEMBL3104628 Ki = 4500.0 nM 5.35
CHEMBL3104627 Ki = 13500.0 nM 4.87