Assay Detail
Binding
CHEMBL3107776
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of 5-LOX-mediated LTB4 production in human neutrophils using arachidonic acid as substrate preincubated for 10 mins followed by substrate addition measured after 8 mins by enzyme immunoassay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Inhibition of LOX by flavonoids: a structure-activity relationship study.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 5.64 | 5.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL151 | LUTEOLIN | 2.0 | 1 | 5.80 |
| CHEMBL222556 | — | — | 1 | 5.77 |
| CHEMBL301624 | — | — | 1 | 5.70 |
| CHEMBL243677 | — | — | 1 | 5.54 |
| CHEMBL50 | QUERCETIN | 3.0 | 1 | 5.40 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL151 | LUTEOLIN | IC50 | = | 1600.0 | nM | 5.80 |
| CHEMBL222556 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL301624 | — | IC50 | = | 2000.0 | nM | 5.70 |
| CHEMBL243677 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL50 | QUERCETIN | IC50 | = | 4000.0 | nM | 5.40 |