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Assay Detail

CHEMBL3111580

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GST-tagged recombinant human VEGFR2 expressed in Sf9 cells by radiometric assay
11
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A development of chimeric VEGFR2 TK inhibitor based on two ligand conformers from PDB: 1Y6A complex--medicinal chemistry consequences of a TKs analysis.
Lintnerová L, García-Caballero M, Gregáň F, Melicherčík M, Quesada AR, Dobiaš J, Lác J, Sališová M, Boháč A.
Eur J Med Chem (2014) 72 : 146 -159
PubMed 24368209 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.39 7.90
Inhibition 4 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1200485 SORAFENIB TOSYLATE 4.0 1 7.90
CHEMBL3109274 2 7.82
CHEMBL194176 1 7.77
CHEMBL3109278 1 7.51
CHEMBL3109275 2 7.29
CHEMBL3109276 2 6.77
CHEMBL3109277 2 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1200485 SORAFENIB TOSYLATE IC50 = 12.5 nM 7.90
CHEMBL3109274 IC50 = 15.1 nM 7.82
CHEMBL194176 IC50 = 17.1 nM 7.77
CHEMBL3109278 IC50 = 31.2 nM 7.51
CHEMBL3109275 IC50 = 51.2 nM 7.29
CHEMBL3109276 IC50 = 170.0 nM 6.77
CHEMBL3109277 IC50 = 202.0 nM 6.70
CHEMBL3109277 Inhibition - % -
CHEMBL3109276 Inhibition - % -
CHEMBL3109275 Inhibition - % -
CHEMBL3109274 Inhibition - % -