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Assay Detail

CHEMBL3111908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of G9a (unknown origin) using biotinylated-histone H3(1-21) peptide as substrate after 3 hrs by AlphaLISA assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone-lysine N-methyltransferase EHMT2 (CHEMBL6032)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and development of potent and selective inhibitors of histone methyltransferase g9a.
Sweis RF, Pliushchev M, Brown PJ, Guo J, Li F, Maag D, Petros AM, Soni NB, Tse C, Vedadi M, Michaelides MR, Chiang GG, Pappano WN.
ACS Med Chem Lett (2014) 5 : 205 -209
PubMed 24900801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.91 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3109639 1 9.05
CHEMBL3109631 1 9.00
CHEMBL3109630 1 8.48
CHEMBL3109637 1 8.43
CHEMBL3109634 1 8.32
CHEMBL3109632 1 8.30
CHEMBL3109638 1 7.75
CHEMBL3109633 1 6.82
CHEMBL3109625 1 6.82
CHEMBL3109636 1 6.12
CHEMBL3109635 1 5.87
CHEMBL3109626 1 5.61
CHEMBL3109627 1 5.45
CHEMBL3109640 1 4.89
CHEMBL3109629 1 4.84
CHEMBL3109628 1 4.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3109639 IC50 = 0.9 nM 9.05
CHEMBL3109631 IC50 = 1.0 nM 9.00
CHEMBL3109630 IC50 = 3.3 nM 8.48
CHEMBL3109637 IC50 = 3.7 nM 8.43
CHEMBL3109634 IC50 = 4.8 nM 8.32
CHEMBL3109632 IC50 = 5.0 nM 8.30
CHEMBL3109638 IC50 = 18.0 nM 7.75
CHEMBL3109633 IC50 = 150.0 nM 6.82
CHEMBL3109625 IC50 = 153.0 nM 6.82
CHEMBL3109636 IC50 = 754.0 nM 6.12
CHEMBL3109635 IC50 = 1342.0 nM 5.87
CHEMBL3109626 IC50 = 2460.0 nM 5.61
CHEMBL3109627 IC50 = 3540.0 nM 5.45
CHEMBL3109640 IC50 = 12900.0 nM 4.89
CHEMBL3109629 IC50 = 14400.0 nM 4.84
CHEMBL3109628 IC50 = 18200.0 nM 4.74