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Assay Detail

CHEMBL3112253

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant JMJD2E (unknown origin) incubated for 15 mins prior to substrate addition by AlphaScreen method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific demethylase 4E (CHEMBL1293226)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pan-histone demethylase inhibitors simultaneously targeting Jumonji C and lysine-specific demethylases display high anticancer activities.
Rotili D, Tomassi S, Conte M, Benedetti R, Tortorici M, Ciossani G, Valente S, Marrocco B, Labella D, Novellino E, Mattevi A, Altucci L, Tumber A, Yapp C, King ON, Hopkinson RJ, Kawamura A, Schofield CJ, Mai A.
J Med Chem (2014) 57 : 42 -55
PubMed 24325601 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.55 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1230640 1 6.52
CHEMBL3108958 1 6.38
CHEMBL3108954 1 5.46
CHEMBL3108952 1 5.41
CHEMBL3108956 1 5.30
CHEMBL3108955 1 5.29
CHEMBL3108953 1 5.26
CHEMBL3108959 1 4.80
CHEMBL1255743 TRANYLCYPROMINE HYDROCHLORIDE 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1230640 IC50 = 300.0 nM 6.52
CHEMBL3108958 IC50 = 420.0 nM 6.38
CHEMBL3108954 IC50 = 3500.0 nM 5.46
CHEMBL3108952 IC50 = 3900.0 nM 5.41
CHEMBL3108956 IC50 = 5000.0 nM 5.30
CHEMBL3108955 IC50 = 5100.0 nM 5.29
CHEMBL3108953 IC50 = 5500.0 nM 5.26
CHEMBL3108959 IC50 = 16000.0 nM 4.80
CHEMBL1255743 TRANYLCYPROMINE HYDROCHLORIDE IC50 > 100000.0 nM -