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Assay Detail

CHEMBL3119092

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant wild-type PAD4 (unknown origin) using N-alpha-Benzoyl-L-arginine ethyl ester as substrate preincubated for 15 mins followed by substrate addition measured after 15 mins by COLDER assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein-arginine deiminase type-4 (CHEMBL6111)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Insights into the mechanism of streptonigrin-induced protein arginine deiminase inactivation.
Dreyton CJ, Anderson ED, Subramanian V, Boger DL, Thompson PR.
Bioorg Med Chem (2014) 22 : 1362 -1369
PubMed 24440480 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.02 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL58150 1 6.62
CHEMBL3113470 1 6.25
CHEMBL57818 1 6.19
CHEMBL60908 1 6.00
CHEMBL11417 STREPTONIGRIN 2.0 1 5.60
CHEMBL1714002 1 5.48

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL58150 IC50 = 240.0 nM 6.62
CHEMBL3113470 IC50 = 560.0 nM 6.25
CHEMBL57818 IC50 = 640.0 nM 6.19
CHEMBL60908 IC50 = 1000.0 nM 6.00
CHEMBL11417 STREPTONIGRIN IC50 = 2500.0 nM 5.60
CHEMBL1714002 IC50 = 3300.0 nM 5.48