Assay Detail
Binding
CHEMBL3123986
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at muscarinic M2 receptor in albino guinea pig left atrium assessed as stimulation of electrically-induced response
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Cavia porcellus |
| Tissue | Cardiac atrium |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Bis(ammonio)alkane-type agonists of muscarinic acetylcholine receptors: synthesis, in vitro functional characterization, and in vivo evaluation of their analgesic activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 10 | 7.80 | 10.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL3121473 | — | — | 1 | 10.10 |
| CHEMBL3121479 | — | — | 1 | 8.15 |
| CHEMBL3121478 | — | — | 1 | 8.11 |
| CHEMBL3121475 | — | — | 1 | 7.91 |
| CHEMBL3121476 | — | — | 1 | 7.85 |
| CHEMBL3120178 | — | — | 1 | 7.73 |
| CHEMBL3121480 | — | — | 1 | 7.67 |
| CHEMBL3121477 | — | — | 1 | 7.42 |
| CHEMBL3121474 | — | — | 1 | 7.18 |
| CHEMBL1482 | BETHANECHOL | 4.0 | 1 | 5.85 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL3121473 | — | EC50 | = | 0.07943 | nM | 10.10 |
| CHEMBL3121479 | — | EC50 | = | 7.079 | nM | 8.15 |
| CHEMBL3121478 | — | EC50 | = | 7.762 | nM | 8.11 |
| CHEMBL3121475 | — | EC50 | = | 12.3 | nM | 7.91 |
| CHEMBL3121476 | — | EC50 | = | 14.13 | nM | 7.85 |
| CHEMBL3120178 | — | EC50 | = | 18.62 | nM | 7.73 |
| CHEMBL3121480 | — | EC50 | = | 21.38 | nM | 7.67 |
| CHEMBL3121477 | — | EC50 | = | 38.02 | nM | 7.42 |
| CHEMBL3121474 | — | EC50 | = | 66.07 | nM | 7.18 |
| CHEMBL1482 | BETHANECHOL | EC50 | = | 1412.54 | nM | 5.85 |