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Assay Detail

CHEMBL3124475

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of electric eel AChE using acetylthiocholine iodide as substrate preincubated for 20 mins followed by substrate addition measured after 5 mins by Ellman's method
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

1,2,3,4-Tetrahydrobenzo[h][1,6]naphthyridines as a new family of potent peripheral-to-midgorge-site inhibitors of acetylcholinesterase: synthesis, pharmacological evaluation and mechanistic studies.
Di Pietro O, Viayna E, Vicente-García E, Bartolini M, Ramón R, Juárez-Jiménez J, Clos MV, Pérez B, Andrisano V, Luque FJ, Lavilla R, Muñoz-Torrero D.
Eur J Med Chem (2014) 73 : 141 -152
PubMed 24389509 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.92 7.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3122167 1 7.34
CHEMBL3122166 1 6.83
CHEMBL3122150 1 6.83
CHEMBL3122147 1 6.55
CHEMBL3122168 1 6.27
CHEMBL3122145 1 5.71
CHEMBL3122169 1 5.67
CHEMBL3122170 1 5.28
CHEMBL3122149 1 5.26
CHEMBL3122172 1 5.20
CHEMBL3122146 1 5.18
CHEMBL3120185 1 4.87
CHEMBL345124 PROPIDIUM 1 -
CHEMBL95 TACRINE 4.0 1 -
CHEMBL3122148 1 -
CHEMBL3122171 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3122167 IC50 = 46.0 nM 7.34
CHEMBL3122166 IC50 = 148.0 nM 6.83
CHEMBL3122150 IC50 = 147.0 nM 6.83
CHEMBL3122147 IC50 = 281.0 nM 6.55
CHEMBL3122168 IC50 = 532.0 nM 6.27
CHEMBL3122145 IC50 = 1970.0 nM 5.71
CHEMBL3122169 IC50 = 2150.0 nM 5.67
CHEMBL3122170 IC50 = 5210.0 nM 5.28
CHEMBL3122149 IC50 = 5480.0 nM 5.26
CHEMBL3122172 IC50 = 6330.0 nM 5.20
CHEMBL3122146 IC50 = 6620.0 nM 5.18
CHEMBL3120185 IC50 = 13600.0 nM 4.87
CHEMBL345124 PROPIDIUM IC50 - -
CHEMBL95 TACRINE IC50 - -
CHEMBL3122148 IC50 > 30000.0 nM -
CHEMBL3122171 IC50 > 30000.0 nM -