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Assay Detail

CHEMBL3128372

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 20s constitutive proteasome beta 2 subunit trypsin-like activity in rabbit erythrocytes using Boc-LRR-AMC as substrate incubated for 15 mins prior to substrate addition measured after 45 mins by fluorescence assay
33
Total Activities
33
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

New C(4)- and C(1)-derivatives of furo[3,4-c]pyridine-3-ones and related compounds: evidence for site-specific inhibition of the constitutive proteasome and its immunoisoform.
Hovhannisyan A, Pham TH, Bouvier D, Piroyan A, Dufau L, Qin L, Cheng Y, Melikyan G, Reboud-Ravaux M, Bouvier-Durand M.
Bioorg Med Chem Lett (2014) 24 : 1571 -1580
PubMed 24534487 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 33 4.84 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL371405 MARIZOMIB 3.0 1 7.68
CHEMBL3128155 1 5.06
CHEMBL3128248 1 5.00
CHEMBL3128249 1 4.71
CHEMBL3128149 1 4.32
CHEMBL3128167 1 4.25
CHEMBL3128156 1 4.23
CHEMBL3128147 1 4.22
CHEMBL3128257 1 4.06
CHEMBL3128148 1 -
CHEMBL3128150 1 -
CHEMBL3128151 1 -
CHEMBL3128152 1 -
CHEMBL3128153 1 -
CHEMBL3128154 1 -
CHEMBL3128157 1 -
CHEMBL3128158 1 -
CHEMBL3128159 1 -
CHEMBL3128161 1 -
CHEMBL3128254 1 -
CHEMBL3128146 1 -
CHEMBL3128145 1 -
CHEMBL3128144 1 -
CHEMBL3128143 1 -
CHEMBL3128140 1 -
CHEMBL3128255 1 -
CHEMBL3128256 1 -
CHEMBL3128253 1 -
CHEMBL3128260 1 -
CHEMBL3128258 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL371405 MARIZOMIB IC50 = 21.0 nM 7.68
CHEMBL3128155 IC50 = 8700.0 nM 5.06
CHEMBL3128248 IC50 = 9900.0 nM 5.00
CHEMBL3128249 IC50 = 19400.0 nM 4.71
CHEMBL3128149 IC50 = 48100.0 nM 4.32
CHEMBL3128167 IC50 = 56000.0 nM 4.25
CHEMBL3128156 IC50 = 59000.0 nM 4.23
CHEMBL3128147 IC50 = 60800.0 nM 4.22
CHEMBL3128257 IC50 = 88000.0 nM 4.06
CHEMBL3128150 IC50 - -
CHEMBL3124973 IC50 - -
CHEMBL3128151 IC50 - -
CHEMBL3128152 IC50 - -
CHEMBL3128153 IC50 - -
CHEMBL3128154 IC50 - -
CHEMBL3128157 IC50 - -
CHEMBL3128158 IC50 - -
CHEMBL3128159 IC50 - -
CHEMBL3128161 IC50 - -
CHEMBL3128164 IC50 - -
CHEMBL3128165 IC50 - -
CHEMBL3128148 IC50 - -
CHEMBL3128254 IC50 - -
CHEMBL3128146 IC50 - -
CHEMBL3128145 IC50 - -
CHEMBL3128144 IC50 - -
CHEMBL3128143 IC50 - -
CHEMBL3128140 IC50 - -
CHEMBL3128255 IC50 - -
CHEMBL3128256 IC50 - -
CHEMBL3128253 IC50 - -
CHEMBL3128260 IC50 - -
CHEMBL3128258 IC50 - -