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Assay Detail

CHEMBL3128638

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SGLT2 (unknown origin) expressed in HEK293 cells using 2-NBDG as substrate incubated for 30 mins prior to substrate addition measured after 30 mins by nonradioactive fluorescence glucose uptake assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of novel l-rhamnose derived acyclic C-nucleosides with substituted 1,2,3-triazole core as potent sodium-glucose co-transporter (SGLT) inhibitors.
Putapatri SR, Kanwal A, Banerjee SK, Kantevari S.
Bioorg Med Chem Lett (2014) 24 : 1528 -1531
PubMed 24556379 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.74 7.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL245067 PHLORIZIN 1 7.23
CHEMBL3125147 1 6.90
CHEMBL3125151 1 6.87
CHEMBL3125155 1 6.83
CHEMBL3125154 1 6.83
CHEMBL3125143 1 6.82
CHEMBL3125142 1 6.82
CHEMBL3125152 1 6.79
CHEMBL3125146 1 6.76
CHEMBL3125141 1 6.73
CHEMBL3125144 1 6.72
CHEMBL3125153 1 6.67
CHEMBL3125149 1 6.64
CHEMBL3125156 1 6.51
CHEMBL3125145 1 6.43
CHEMBL3125148 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL245067 PHLORIZIN IC50 = 58.6 nM 7.23
CHEMBL3125147 IC50 = 125.9 nM 6.90
CHEMBL3125151 IC50 = 134.7 nM 6.87
CHEMBL3125155 IC50 = 146.9 nM 6.83
CHEMBL3125154 IC50 = 149.1 nM 6.83
CHEMBL3125143 IC50 = 151.8 nM 6.82
CHEMBL3125142 IC50 = 150.0 nM 6.82
CHEMBL3125152 IC50 = 163.2 nM 6.79
CHEMBL3125146 IC50 = 175.0 nM 6.76
CHEMBL3125141 IC50 = 185.8 nM 6.73
CHEMBL3125144 IC50 = 190.3 nM 6.72
CHEMBL3125153 IC50 = 214.2 nM 6.67
CHEMBL3125149 IC50 = 227.0 nM 6.64
CHEMBL3125156 IC50 = 311.0 nM 6.51
CHEMBL3125145 IC50 = 367.0 nM 6.43
CHEMBL3125148 IC50 = 458.0 nM 6.34