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Assay Detail

CHEMBL3129688

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli RNAP assessed as incorporation of [5,6-3H]UTP preincubated for 10 mins followed by DNA template and ATP, CTP, GTP, UTP, [5,6-3H]UTP addition measured after 10 mins by scintillation counting analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Benzamidobenzoic acids as potent PqsD inhibitors for the treatment of Pseudomonas aeruginosa infections.
Hinsberger S, de Jong JC, Groh M, Haupenthal J, Hartmann RW.
Eur J Med Chem (2014) 76 : 343 -351
PubMed 24589489 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 4.61 5.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3127032 1 5.05
CHEMBL3127030 1 5.00
CHEMBL3127031 1 4.89
CHEMBL3127034 1 4.46
CHEMBL3127038 1 4.13
CHEMBL2413673 1 4.11
CHEMBL3127029 1 -
CHEMBL3127025 1 -
CHEMBL3127035 1 -
CHEMBL3127036 1 -
CHEMBL3127037 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3127032 IC50 = 9000.0 nM 5.05
CHEMBL3127030 IC50 = 10000.0 nM 5.00
CHEMBL3127031 IC50 = 13000.0 nM 4.89
CHEMBL3127034 IC50 = 35000.0 nM 4.46
CHEMBL3127038 IC50 = 74000.0 nM 4.13
CHEMBL2413673 IC50 = 78000.0 nM 4.11
CHEMBL3127029 IC50 = 161000.0 nM -
CHEMBL3127025 IC50 = 110000.0 nM -
CHEMBL3127035 IC50 - -
CHEMBL3127036 IC50 - -
CHEMBL3127037 IC50 - -