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Assay Detail

CHEMBL3131492

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Electric eel AChE assessed as amount of thiocholine released using acetylthiocholine iodide as substrate after 6 mins by Ellman's method
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Electrophorus electricus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL4078)
Type SINGLE PROTEIN
Organism Electrophorus electricus

Publication

Chemical composition of the bark of Tetrapterys mucronata and identification of acetylcholinesterase inhibitory constituents.
Queiroz MM, Queiroz EF, Zeraik ML, Ebrahimi SN, Marcourt L, Cuendet M, Castro-Gamboa I, Hamburger M, da Silva Bolzani V, Wolfender JL.
J Nat Prod (2014) 77 : 650 -656
PubMed 24521095 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.31 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL95 TACRINE 4.0 1 7.05
CHEMBL659 GALANTAMINE 4.0 1 5.62
CHEMBL416526 BUFOTENINE 1 4.94
CHEMBL3125442 1 4.93
CHEMBL3125443 1 4.90
CHEMBL58579 1 4.90
CHEMBL7257 MEBUFOTENIN 2.0 1 4.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL95 TACRINE IC50 = 90.0 nM 7.05
CHEMBL659 GALANTAMINE IC50 = 2400.0 nM 5.62
CHEMBL416526 BUFOTENINE IC50 = 11400.0 nM 4.94
CHEMBL3125442 IC50 = 11700.0 nM 4.93
CHEMBL3125443 IC50 = 12700.0 nM 4.90
CHEMBL58579 IC50 = 12500.0 nM 4.90
CHEMBL7257 MEBUFOTENIN IC50 = 14000.0 nM 4.85