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Assay Detail

CHEMBL3131493

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant 5-lipoxygenase using arachidonic acid as substrate preincubated for 10 mins before substrate addition measured after 10 mins by HPLC analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Polyunsaturated fatty acid 5-lipoxygenase (CHEMBL215)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

One-step semisynthesis of oleacein and the determination as a 5-lipoxygenase inhibitor.
Vougogiannopoulou K, Lemus C, Halabalaki M, Pergola C, Werz O, Smith AB, Michel S, Skaltsounis L, Deguin B.
J Nat Prod (2014) 77 : 441 -445
PubMed 24568174 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 5.53 6.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL93 ZILEUTON 4.0 1 6.00
CHEMBL3125444 1 5.70
CHEMBL1950045 HYDROXYTYROSOL 3.0 1 4.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL93 ZILEUTON IC50 = 1000.0 nM 6.00
CHEMBL3125444 IC50 = 2000.0 nM 5.70
CHEMBL1950045 HYDROXYTYROSOL IC50 = 13000.0 nM 4.89