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Assay Detail

CHEMBL3131692

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-epibatidine from alpha4beta2* nAChR in Sprague-Dawley rat cerebral cortex after 4 hrs by liquid scintillation counting analysis
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Synthesis, nicotinic acetylcholine receptor binding, and antinociceptive properties of 2'-fluoro-3'-(substituted pyridinyl)-7-deschloroepibatidine analogues.
Ondachi PW, Castro AH, Bartkowiak JM, Luetje CW, Damaj MI, Mascarella SW, Navarro HA, Carroll FI.
J Med Chem (2014) 57 : 836 -848
PubMed 24428686 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 9.85 10.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6623 EPIBATIDINE 1 10.59
CHEMBL3128035 1 10.40
CHEMBL3128037 1 10.31
CHEMBL3128038 1 10.20
CHEMBL3128032 1 10.17
CHEMBL3128031 1 9.92
CHEMBL1076903 VARENICLINE 4.0 1 9.92
CHEMBL3128028 1 9.89
CHEMBL3128034 1 9.89
CHEMBL3128039 1 9.60
CHEMBL3128036 1 9.46
CHEMBL3128033 1 8.93
CHEMBL3 NICOTINE 4.0 1 8.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6623 EPIBATIDINE Ki = 0.026 nM 10.59
CHEMBL3128035 Ki = 0.04 nM 10.40
CHEMBL3128037 Ki = 0.049 nM 10.31
CHEMBL3128038 Ki = 0.063 nM 10.20
CHEMBL3128032 Ki = 0.067 nM 10.17
CHEMBL3128031 Ki = 0.12 nM 9.92
CHEMBL1076903 VARENICLINE Ki = 0.12 nM 9.92
CHEMBL3128028 Ki = 0.13 nM 9.89
CHEMBL3128034 Ki = 0.13 nM 9.89
CHEMBL3128039 Ki = 0.25 nM 9.60
CHEMBL3128036 Ki = 0.35 nM 9.46
CHEMBL3128033 Ki = 1.18 nM 8.93
CHEMBL3 NICOTINE Ki = 1.5 nM 8.82