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Assay Detail

CHEMBL3134675

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human caspase-7 expressed in Escherichia coli using Ac-DEVD-AMC as substrate preincubated for 30 mins before substrate addition measured after 2 hrs
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Caspase-7 (CHEMBL3468)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A new class of fluorinated 5-pyrrolidinylsulfonyl isatin caspase inhibitors for PET imaging of apoptosis
Krause-Heuer AM, Howell NR, Matesic L, Dhand G, Young EL, Burgess L, Jiang CD, Lengkeek NA, Fookes CJR, Pham TQ, Sobrio F, Greguric I, Fraser BH
Medchemcomm (2013) 4 : 347 -352
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.73 8.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL215480 1 8.34
CHEMBL3133258 1 8.27
CHEMBL213759 1 8.23
CHEMBL217777 1 8.21
CHEMBL3133259 1 8.02
CHEMBL3133148 1 7.95
CHEMBL3133255 1 7.85
CHEMBL3133254 1 7.85
CHEMBL3133253 1 7.80
CHEMBL3133147 1 7.78
CHEMBL3133146 1 7.70
CHEMBL3133252 1 7.69
CHEMBL3133257 1 7.66
CHEMBL3133256 1 7.62
CHEMBL3133149 1 7.60
CHEMBL3133251 1 7.38
CHEMBL3133150 1 5.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL215480 IC50 = 4.6 nM 8.34
CHEMBL3133258 IC50 = 5.4 nM 8.27
CHEMBL213759 IC50 = 5.9 nM 8.23
CHEMBL217777 IC50 = 6.1 nM 8.21
CHEMBL3133259 IC50 = 9.6 nM 8.02
CHEMBL3133148 IC50 = 11.2 nM 7.95
CHEMBL3133255 IC50 = 14.0 nM 7.85
CHEMBL3133254 IC50 = 14.2 nM 7.85
CHEMBL3133253 IC50 = 15.9 nM 7.80
CHEMBL3133147 IC50 = 16.5 nM 7.78
CHEMBL3133146 IC50 = 19.8 nM 7.70
CHEMBL3133252 IC50 = 20.4 nM 7.69
CHEMBL3133257 IC50 = 21.8 nM 7.66
CHEMBL3133256 IC50 = 24.0 nM 7.62
CHEMBL3133149 IC50 = 25.3 nM 7.60
CHEMBL3133251 IC50 = 41.4 nM 7.38
CHEMBL3133150 IC50 = 3512.0 nM 5.45