Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3134683

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of calcineurin in human Jurkat cells assessed as inhibition of NFAT-mediated IL-2 production after 8 hrs by beta-lactamase reporter gene assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Jurkat
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Novel FK506 and FK520 analogues via mutasynthesis: mutasynthon scope and product characteristics
Moss SJ, Stanley-Smith AE, Schell U, Coates NJ, Foster TA, Gaisser S, Gregory MA, Martin CJ, Nur-e-Alam M, Piraee M, Radzom M, Suthar D, Thexton DG, Warneck TD, Zhang M, Wilkinson B
Medchemcomm (2013) 4 : 324 -331
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 9.76 10.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS 4.0 1 10.25
CHEMBL3133264 1 10.14
CHEMBL3133263 1 10.09
CHEMBL3133260 1 9.84
CHEMBL8597 ASCOMYCIN 1 9.69
CHEMBL3133261 1 9.60
CHEMBL3133266 1 9.55
CHEMBL3133262 1 9.36
CHEMBL3133265 1 9.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL269732 TACROLIMUS ANHYDROUS IC50 = 0.056 nM 10.25
CHEMBL3133264 IC50 = 0.072 nM 10.14
CHEMBL3133263 IC50 = 0.082 nM 10.09
CHEMBL3133260 IC50 = 0.145 nM 9.84
CHEMBL8597 ASCOMYCIN IC50 = 0.205 nM 9.69
CHEMBL3133261 IC50 = 0.249 nM 9.60
CHEMBL3133266 IC50 = 0.283 nM 9.55
CHEMBL3133262 IC50 = 0.433 nM 9.36
CHEMBL3133265 IC50 = 0.461 nM 9.34