Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL3223375

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NEP using N-Dansyl-D-Ala-Gly-p-nitro-Phe-Gly as substrate preincubated for 10 mins followed by substrate addition measured after 2 hrs by spectrophotometric analysis
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Neprilysin (CHEMBL1944)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel selective inhibitors of neutral endopeptidase: discovery by screening and hit-to-lead optimisation
El Bakali J, Maingot L, Dumont J, Host H, Hocine A, Cousaert N, Dassonneville S, Leroux F, Deprez B, Deprez-Poulain R
Medchemcomm (2012) 3 : 469 -474
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.91 8.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL10247 THIORPHAN -1.0 1 8.89
CHEMBL3221965 1 7.20
CHEMBL3221963 1 6.10
CHEMBL3221958 1 5.60
CHEMBL3222111 1 5.20
CHEMBL3221959 1 4.30
CHEMBL3221954 1 4.10
CHEMBL3221955 1 -
CHEMBL3221956 1 -
CHEMBL3221957 1 -
CHEMBL3221960 1 -
CHEMBL3221961 1 -
CHEMBL3221962 1 -
CHEMBL3221964 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL10247 THIORPHAN IC50 = 1.3 nM 8.89
CHEMBL3221965 IC50 = 63.1 nM 7.20
CHEMBL3221963 IC50 = 794.33 nM 6.10
CHEMBL3221958 IC50 = 2511.89 nM 5.60
CHEMBL3222111 IC50 = 6309.57 nM 5.20
CHEMBL3221959 IC50 = 50118.72 nM 4.30
CHEMBL3221954 IC50 = 79432.82 nM 4.10
CHEMBL3221955 IC50 > 1000000.0 nM -
CHEMBL3221956 IC50 > 1000000.0 nM -
CHEMBL3221957 IC50 > 1000000.0 nM -
CHEMBL3221960 IC50 > 1000000.0 nM -
CHEMBL3221961 IC50 > 1000000.0 nM -
CHEMBL3221962 IC50 > 1000000.0 nM -
CHEMBL3221964 IC50 > 1000000.0 nM -