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Assay Detail

CHEMBL3223666

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of recombinant human glucokinase assessed as formation of glucose-6-phosphate by G6PDH/NADP coupled assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Designing glucokinase activators with reduced hypoglycemia risk: discovery of N,N-dimethyl-5-(2-methyl-6-((5-methylpyrazin-2-yl)-carbamoyl)benzofuran-4-yloxy)pyrimidine-2-carboxamide as a clinical candidate for the treatment of type 2 diabetes mellitus
Pfefferkorn JA, Guzman-Perez A, Oates PJ, Litchfield J, Aspnes G, Basak A, Benbow J, Berliner MA, Bian J, Choi C, Freeman-Cook K, Corbett JW, Didiuk M, Dunetz JR, Filipski KJ, Hungerford WM, Jones CS, Karki K, Ling A, Li J, Patel L, Perreault C, Risley H, Saenz J, Song W, Tu M, Aiello R, Atkinson K, Barucci N, Beebe D, Bourassa P, Bourbounais F, Brodeur AM, Burbey R, Chen J, D'Aquila T, Derksen DR, Haddish-Berhane N, Huang C, Landro J, Lee Lapworth A, MacDougall M, Perregaux D, Pettersen J, Robertson A, Tan B, Treadway JL, Liu S, Qiu X, Knafels J, Ammirati M, Song X, DaSilva-Jardine P, Liras S, Sweet L, Rolph TP
Medchemcomm (2011) 2 : 828 -839
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 32 6.59 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3218477 1 7.57
CHEMBL499520 1 7.43
CHEMBL3218488 1 7.19
CHEMBL1783735 1 7.18
CHEMBL3218487 1 7.07
CHEMBL3218478 1 7.06
CHEMBL3218482 1 7.04
CHEMBL575726 1 6.91
CHEMBL3218497 1 6.90
CHEMBL3218485 1 6.82
CHEMBL3218498 1 6.78
CHEMBL2165615 NERIGLIATIN 2.0 1 6.73
CHEMBL2165616 1 6.67
CHEMBL3218499 1 6.59
CHEMBL3218489 1 6.58
CHEMBL1096435 Ro-281675 1 6.48
CHEMBL3218500 1 6.48
CHEMBL1783734 PIRAGLIATIN 2.0 1 6.44
CHEMBL3218490 1 6.41
CHEMBL3218496 1 6.41
CHEMBL3218483 1 6.32
CHEMBL3218495 1 6.32
CHEMBL3218491 1 6.15
CHEMBL3218480 1 6.06
CHEMBL3218494 1 5.66
CHEMBL3218481 1 5.51
CHEMBL3218479 1 5.18
CHEMBL3218501 1 -
CHEMBL3218493 1 -
CHEMBL3218492 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3218477 EC50 = 27.0 nM 7.57
CHEMBL499520 EC50 = 37.0 nM 7.43
CHEMBL3218488 EC50 = 64.0 nM 7.19
CHEMBL1783735 EC50 = 66.0 nM 7.18
CHEMBL3218487 EC50 = 85.0 nM 7.07
CHEMBL3218478 EC50 = 88.0 nM 7.06
CHEMBL3218482 EC50 = 92.0 nM 7.04
CHEMBL575726 EC50 = 122.0 nM 6.91
CHEMBL3218497 EC50 = 127.0 nM 6.90
CHEMBL3218485 EC50 = 153.0 nM 6.82
CHEMBL3218498 EC50 = 167.0 nM 6.78
CHEMBL2165615 NERIGLIATIN EC50 = 188.0 nM 6.73
CHEMBL2165616 EC50 = 212.0 nM 6.67
CHEMBL3218499 EC50 = 254.0 nM 6.59
CHEMBL3218489 EC50 = 261.0 nM 6.58
CHEMBL3218500 EC50 = 331.0 nM 6.48
CHEMBL1096435 Ro-281675 EC50 = 334.0 nM 6.48
CHEMBL1783734 PIRAGLIATIN EC50 = 364.0 nM 6.44
CHEMBL3218490 EC50 = 393.0 nM 6.41
CHEMBL3218496 EC50 = 391.0 nM 6.41
CHEMBL3218483 EC50 = 480.0 nM 6.32
CHEMBL3218495 EC50 = 477.0 nM 6.32
CHEMBL3218491 EC50 = 705.0 nM 6.15
CHEMBL3218480 EC50 = 870.0 nM 6.06
CHEMBL3218494 EC50 = 2170.0 nM 5.66
CHEMBL3218481 EC50 = 3061.0 nM 5.51
CHEMBL3218479 EC50 = 6610.0 nM 5.18
CHEMBL3218501 EC50 > 100000.0 nM -
CHEMBL3218493 EC50 > 5000.0 nM -
CHEMBL3218492 EC50 > 5000.0 nM -
CHEMBL3218486 EC50 > 5000.0 nM -
CHEMBL3218484 EC50 > 20.0 nM -