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Assay Detail

CHEMBL3225083

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CYP11B2 (unknown origin) expressed in Chinese hamster V79MZ cells using [1,2-3H]-11-deoxycorticosterone as substrate preincubated for 60 mins followed by substrate addition measured after 50 mins by HPLC analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type V79MZ
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 11B2, mitochondrial (CHEMBL2722)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of imidazolylmethylacridones as cytochrome P-450 enzymes inhibitors
Abadi AH, Abou-Seri SM, Hu Q, Negri M, Hartmann RW
Medchemcomm (2012) 3 : 663 -666
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.14 7.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL934 METYRAPONE 4.0 1 7.14
CHEMBL3219070 1 -
CHEMBL3219071 1 -
CHEMBL1444 LETROZOLE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL934 METYRAPONE IC50 = 71.8 nM 7.14
CHEMBL3219070 IC50 - -
CHEMBL3219071 IC50 - -
CHEMBL1444 LETROZOLE IC50 - -