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Assay Detail

CHEMBL3226233

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of 20S proteasome beta5 subunit in human erythrocytes using Ac-WLA-AMC as substrate by fluorescence assay
32
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Proteasome Macropain subunit MB1 (CHEMBL4662)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of a series of dipeptides with a P3 -neopentyl asparagine residue as non-covalent inhibitors of the chymotrypsin-like activity of human 20S proteasome
Blackburn C, Barrett C, Blank JL, Bruzzese FJ, Bump N, Dick LR, Fleming P, Garcia K, Hales P, Jones M, Liu JX, Nagayoshi M, Sappal DS, Sintchak MD, Tsu C, Xia C, Zhou X, Gigstad KM
Medchemcomm (2012) 3 : 710 -719
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 32 7.96 8.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3218837 1 8.92
CHEMBL325041 BORTEZOMIB 3.0 1 8.70
CHEMBL3218834 1 8.68
CHEMBL2141296 IXAZOMIB 3.0 1 8.52
CHEMBL3218831 1 8.52
CHEMBL3218833 1 8.43
CHEMBL3218836 1 8.41
CHEMBL3218830 1 8.40
CHEMBL3218835 1 8.40
CHEMBL3218828 1 8.33
CHEMBL3218827 1 8.32
CHEMBL3218824 1 8.27
CHEMBL3218826 1 8.17
CHEMBL3218843 1 8.14
CHEMBL3218823 1 8.08
CHEMBL3218832 1 7.96
CHEMBL3218844 1 7.94
CHEMBL3218822 1 7.92
CHEMBL3218829 1 7.92
CHEMBL1269181 1 7.80
CHEMBL3218840 1 7.80
CHEMBL3218825 1 7.66
CHEMBL3218839 1 7.65
CHEMBL3218841 1 7.64
CHEMBL3218821 1 7.60
CHEMBL3218838 1 7.48
CHEMBL3218820 1 7.30
CHEMBL3218845 1 7.03
CHEMBL3218842 1 6.80
CHEMBL3218819 1 6.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3218837 IC50 = 1.2 nM 8.92
CHEMBL325041 BORTEZOMIB IC50 = 2.0 nM 8.70
CHEMBL3218834 IC50 = 2.1 nM 8.68
CHEMBL2141296 IXAZOMIB IC50 = 3.0 nM 8.52
CHEMBL3218831 IC50 = 3.0 nM 8.52
CHEMBL3218833 IC50 = 3.7 nM 8.43
CHEMBL3218836 IC50 = 3.9 nM 8.41
CHEMBL3218830 IC50 = 4.0 nM 8.40
CHEMBL3218835 IC50 = 4.0 nM 8.40
CHEMBL3218828 IC50 = 4.7 nM 8.33
CHEMBL3218827 IC50 = 4.8 nM 8.32
CHEMBL3218824 IC50 = 5.4 nM 8.27
CHEMBL3218826 IC50 = 6.8 nM 8.17
CHEMBL3218843 IC50 = 7.3 nM 8.14
CHEMBL3218823 IC50 = 8.4 nM 8.08
CHEMBL3218832 IC50 = 11.0 nM 7.96
CHEMBL3218844 IC50 = 11.6 nM 7.94
CHEMBL3218822 IC50 = 12.0 nM 7.92
CHEMBL3218829 IC50 = 12.0 nM 7.92
CHEMBL1269181 IC50 = 16.0 nM 7.80
CHEMBL3218840 IC50 = 16.0 nM 7.80
CHEMBL3218825 IC50 = 22.0 nM 7.66
CHEMBL3218839 IC50 = 22.6 nM 7.65
CHEMBL3218841 IC50 = 23.0 nM 7.64
CHEMBL3218821 IC50 = 25.0 nM 7.60
CHEMBL3218838 IC50 = 33.3 nM 7.48
CHEMBL3218820 IC50 = 50.0 nM 7.30
CHEMBL3218845 IC50 = 93.0 nM 7.03
CHEMBL3218842 IC50 = 160.0 nM 6.80
CHEMBL3218819 IC50 = 910.0 nM 6.04
CHEMBL3218817 IC50 > 1000.0 nM -
CHEMBL3218818 IC50 > 1000.0 nM -