Assay Detail

CHEMBL3227735

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding

Inhibition of Aquifex aeolicus IspH expressed in Escherichia coli BL21(DE3) using HMBPP as substrate preincubated for 10 mins followed by substrate addition

ChEMBL ↗

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Target

Unchecked (CHEMBL612545) ↗

Open DB target context

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Unchecked (CHEMBL612545) is the working anchor for this assay review. Loading the live Open DB target snapshot…

Primary target Unchecked

CHEMBL612545

Anchored from Assay target.

Protein identity Unchecked

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Binding
Organism	Aquifex aeolicus
Confidence	0 — Uncurated / Unknown
Curated By	Autocuration

Target

Unchecked (CHEMBL612545) ↗

Type

UNCHECKED

Publication

The MEP pathway and the development of inhibitors as potential anti-infective agents

Hale I, O'Neill PM, Berry NG, Odom A, Sharma R
Medchemcomm (2012) 3 : 418 -433
· DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	1	6.35	6.35

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL2251810	BUT-3-YNYL DIPHOSPHATE	—	1	6.35

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL2251810	BUT-3-YNYL DIPHOSPHATE	IC50	=	450.0	nM	6.35