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Assay Detail

CHEMBL3239336

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Escherichia coli DNA polymerase 3 beta assessed as inhibition of 5-carboxyfluorescein-QLDLF binding by fluorescence polarization-based competition assay
11
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of lead compounds targeting the bacterial sliding clamp using a fragment-based approach.
Yin Z, Whittell LR, Wang Y, Jergic S, Liu M, Harry EJ, Dixon NE, Beck JL, Kelso MJ, Oakley AJ.
J Med Chem (2014) 57 : 2799 -2806
PubMed 24592885 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 3.43 3.94
Ki 5 3.68 4.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3233038 2 4.19
CHEMBL3235575 2 3.70
CHEMBL1466999 2 3.67
CHEMBL106708 2 3.55
CHEMBL3235577 2 3.30
CHEMBL3235576 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3233038 Ki = 64000.0 nM 4.19
CHEMBL3233038 IC50 = 115000.0 nM 3.94
CHEMBL3235575 Ki = 199000.0 nM 3.70
CHEMBL1466999 Ki = 216000.0 nM 3.67
CHEMBL106708 Ki = 280000.0 nM 3.55
CHEMBL3235575 IC50 = 358000.0 nM 3.45
CHEMBL1466999 IC50 = 389000.0 nM 3.41
CHEMBL3235577 Ki = 499000.0 nM 3.30
CHEMBL106708 IC50 = 504000.0 nM 3.30
CHEMBL3235577 IC50 = 899000.0 nM 3.05
CHEMBL3235576 IC50 > 1000000.0 nM -