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Assay Detail

CHEMBL3240083

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of chymotrypsin-like activity of human 20S proteasome using Suc-LLVY-AMC as substrate
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

26S proteasome (CHEMBL2364701)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Structurally novel highly potent proteasome inhibitors created by the structure-based hybridization of nonpeptidic belactosin derivatives and peptide boronates.
Kawamura S, Unno Y, Asai A, Arisawa M, Shuto S.
J Med Chem (2014) 57 : 2726 -2735
PubMed 24524217 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 8.02 8.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3237863 1 8.57
CHEMBL3237862 1 8.41
CHEMBL3237864 1 8.39
CHEMBL3237867 1 8.39
CHEMBL3237873 1 8.32
CHEMBL3237865 1 8.29
CHEMBL3237866 1 8.24
CHEMBL3237860 1 8.24
CHEMBL3237868 1 8.23
CHEMBL325041 BORTEZOMIB 3.0 1 8.15
CHEMBL3237870 1 8.15
CHEMBL3237871 1 8.12
CHEMBL3237874 1 8.11
CHEMBL3237869 1 8.11
CHEMBL3237861 1 7.54
CHEMBL3237872 1 7.27
CHEMBL491636 BELACTOSIN A 1 5.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3237863 IC50 = 2.7 nM 8.57
CHEMBL3237862 IC50 = 3.9 nM 8.41
CHEMBL3237864 IC50 = 4.1 nM 8.39
CHEMBL3237867 IC50 = 4.1 nM 8.39
CHEMBL3237873 IC50 = 4.8 nM 8.32
CHEMBL3237865 IC50 = 5.1 nM 8.29
CHEMBL3237866 IC50 = 5.7 nM 8.24
CHEMBL3237860 IC50 = 5.7 nM 8.24
CHEMBL3237868 IC50 = 5.9 nM 8.23
CHEMBL325041 BORTEZOMIB IC50 = 7.0 nM 8.15
CHEMBL3237870 IC50 = 7.1 nM 8.15
CHEMBL3237871 IC50 = 7.5 nM 8.12
CHEMBL3237874 IC50 = 7.7 nM 8.11
CHEMBL3237869 IC50 = 7.8 nM 8.11
CHEMBL3237861 IC50 = 29.0 nM 7.54
CHEMBL3237872 IC50 = 54.0 nM 7.27
CHEMBL491636 BELACTOSIN A IC50 = 1400.0 nM 5.85