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Assay Detail

CHEMBL3240359

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat brain VGLUT assessed as inhibition of [3H]glutamate uptake into vesicles after 10 mins by scintillation spectrophotometry
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Design, synthesis and biological evaluation of small-azo-dyes as potent Vesicular Glutamate Transporters inhibitors.
Favre-Besse FC, Poirel O, Bersot T, Kim-Grellier E, Daumas S, El Mestikawy S, Acher FC, Pietrancosta N.
Eur J Med Chem (2014) 78 : 236 -247
PubMed 24686010 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.70 7.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1640 TRYPAN BLUE 4.0 1 7.30
CHEMBL1200712 EVANS BLUE 4.0 1 7.06
CHEMBL3238498 1 5.80
CHEMBL3238500 1 5.75
CHEMBL3238484 1 5.68
CHEMBL3238491 1 5.68
CHEMBL311909 CHICAGO SKY BLUE 1 5.52
CHEMBL3238501 1 5.48
CHEMBL3238499 1 5.32
CHEMBL3238488 1 5.24
CHEMBL3238480 1 5.17
CHEMBL76124 1 4.40
CHEMBL3238490 1 -
CHEMBL72332 1 -
CHEMBL3238492 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1640 TRYPAN BLUE IC50 = 50.0 nM 7.30
CHEMBL1200712 EVANS BLUE IC50 = 87.0 nM 7.06
CHEMBL3238498 IC50 = 1600.0 nM 5.80
CHEMBL3238500 IC50 = 1800.0 nM 5.75
CHEMBL3238484 IC50 = 2100.0 nM 5.68
CHEMBL3238491 IC50 = 2100.0 nM 5.68
CHEMBL311909 CHICAGO SKY BLUE IC50 = 3000.0 nM 5.52
CHEMBL3238501 IC50 = 3300.0 nM 5.48
CHEMBL3238499 IC50 = 4800.0 nM 5.32
CHEMBL3238488 IC50 = 5800.0 nM 5.24
CHEMBL3238480 IC50 = 6800.0 nM 5.17
CHEMBL76124 IC50 = 40000.0 nM 4.40
CHEMBL3238490 IC50 > 20000.0 nM -
CHEMBL72332 IC50 = 286000.0 nM -
CHEMBL3238492 IC50 = 288000.0 nM -