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Assay Detail

CHEMBL3240405

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine liver beta-glucuronidase using p-nitrophenyl-beta-D-glucuronide as substrate after 30 mins by spectrophotometry
18
Total Activities
18
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-glucuronidase (CHEMBL4082)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Evaluation of bisindole as potent β-glucuronidase inhibitors: synthesis and in silico based studies.
Khan KM, Rahim F, Wadood A, Taha M, Khan M, Naureen S, Ambreen N, Hussain S, Perveen S, Choudhary MI.
Bioorg Med Chem Lett (2014) 24 : 1825 -1829
PubMed 24602903 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.06 5.79
Inhibition 7 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3237329 1 5.79
CHEMBL2430239 1 5.73
CHEMBL3237336 1 5.55
CHEMBL3237335 1 5.51
CHEMBL3237340 1 5.37
CHEMBL3237330 1 4.74
CHEMBL3237343 1 4.70
CHEMBL3237332 1 4.68
CHEMBL3237334 1 4.67
CHEMBL3237331 1 4.65
CHEMBL1808316 D-SACCHARIC ACID 1,4-LACTONE 1 4.32
CHEMBL2430214 1 -
CHEMBL3237333 1 -
CHEMBL3237337 1 -
CHEMBL3237338 1 -
CHEMBL3237339 1 -
CHEMBL3237341 1 -
CHEMBL3237342 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3237329 IC50 = 1620.0 nM 5.79
CHEMBL2430239 IC50 = 1860.0 nM 5.73
CHEMBL3237336 IC50 = 2800.0 nM 5.55
CHEMBL3237335 IC50 = 3100.0 nM 5.51
CHEMBL3237340 IC50 = 4300.0 nM 5.37
CHEMBL3237330 IC50 = 18400.0 nM 4.74
CHEMBL3237343 IC50 = 19900.0 nM 4.70
CHEMBL3237332 IC50 = 20900.0 nM 4.68
CHEMBL3237334 IC50 = 21500.0 nM 4.67
CHEMBL3237331 IC50 = 22300.0 nM 4.65
CHEMBL1808316 D-SACCHARIC ACID 1,4-LACTONE IC50 = 48400.0 nM 4.32
CHEMBL2430214 Inhibition < 50.0 % -
CHEMBL3237333 Inhibition < 50.0 % -
CHEMBL3237337 Inhibition < 50.0 % -
CHEMBL3237338 Inhibition < 50.0 % -
CHEMBL3237339 Inhibition < 50.0 % -
CHEMBL3237341 Inhibition < 50.0 % -
CHEMBL3237342 Inhibition < 50.0 % -