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Assay Detail

CHEMBL3240702

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of GKRP-GK interaction in mouse hepatocytes assessed as induction of GK translocation from nucleus to cytoplasm incubated for 20 mins prior to glucose addition measured after 40 mins
39
Total Activities
39
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type Hepatocyte
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucokinase regulatory protein (CHEMBL3232700)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Small molecule disruptors of the glucokinase-glucokinase regulatory protein interaction: 3. Structure-activity relationships within the aryl carbinol region of the N-arylsulfonamido-N'-arylpiperazine series.
Nishimura N, Norman MH, Liu L, Yang KC, Ashton KS, Bartberger MD, Chmait S, Chen J, Cupples R, Fotsch C, Helmering J, Jordan SR, Kunz RK, Pennington LD, Poon SF, Siegmund A, Sivits G, Lloyd DJ, Hale C, St Jean DJ.
J Med Chem (2014) 57 : 3094 -3116
PubMed 24611879 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 39 6.44 7.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3237992 1 7.43
CHEMBL3237981 1 7.05
CHEMBL3237980 1 7.01
CHEMBL3237993 1 7.01
CHEMBL3114187 1 6.92
CHEMBL3237986 1 6.89
CHEMBL3237987 1 6.86
CHEMBL3238312 1 6.84
CHEMBL3238314 1 6.81
CHEMBL3238307 1 6.80
CHEMBL3238296 1 6.75
CHEMBL3237985 1 6.70
CHEMBL3114185 1 6.70
CHEMBL3238300 1 6.68
CHEMBL3237984 1 6.66
CHEMBL3237994 1 6.59
CHEMBL3238297 1 6.57
CHEMBL3238306 1 6.53
CHEMBL3238313 1 6.46
CHEMBL3238311 1 6.42
CHEMBL3238308 1 6.42
CHEMBL3238303 1 6.41
CHEMBL3238309 1 6.38
CHEMBL3237983 1 6.27
CHEMBL3238301 1 6.25
CHEMBL3238299 1 6.15
CHEMBL3237991 1 6.03
CHEMBL3238305 1 5.84
CHEMBL3237979 1 5.81
CHEMBL3238304 1 5.69

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3237992 EC50 = 37.0 nM 7.43
CHEMBL3237981 EC50 = 89.0 nM 7.05
CHEMBL3237993 EC50 = 97.0 nM 7.01
CHEMBL3237980 EC50 = 98.0 nM 7.01
CHEMBL3114187 EC50 = 120.0 nM 6.92
CHEMBL3237986 EC50 = 130.0 nM 6.89
CHEMBL3237987 EC50 = 137.0 nM 6.86
CHEMBL3238312 EC50 = 145.0 nM 6.84
CHEMBL3238314 EC50 = 155.0 nM 6.81
CHEMBL3238307 EC50 = 160.0 nM 6.80
CHEMBL3238296 EC50 = 178.0 nM 6.75
CHEMBL3237985 EC50 = 202.0 nM 6.70
CHEMBL3114185 EC50 = 202.0 nM 6.70
CHEMBL3238300 EC50 = 208.0 nM 6.68
CHEMBL3237984 EC50 = 219.0 nM 6.66
CHEMBL3237994 EC50 = 254.0 nM 6.59
CHEMBL3238297 EC50 = 271.0 nM 6.57
CHEMBL3238306 EC50 = 294.0 nM 6.53
CHEMBL3238313 EC50 = 349.0 nM 6.46
CHEMBL3238311 EC50 = 381.0 nM 6.42
CHEMBL3238308 EC50 = 382.0 nM 6.42
CHEMBL3238303 EC50 = 388.0 nM 6.41
CHEMBL3238309 EC50 = 421.0 nM 6.38
CHEMBL3237983 EC50 = 537.0 nM 6.27
CHEMBL3238301 EC50 = 567.0 nM 6.25
CHEMBL3238299 EC50 = 704.0 nM 6.15
CHEMBL3237991 EC50 = 936.0 nM 6.03
CHEMBL3238305 EC50 = 1450.0 nM 5.84
CHEMBL3237979 EC50 = 1560.0 nM 5.81
CHEMBL3238304 EC50 = 2060.0 nM 5.69
CHEMBL3238310 EC50 = 2200.0 nM 5.66
CHEMBL3238302 EC50 = 2220.0 nM 5.65
CHEMBL3237989 EC50 = 4050.0 nM 5.39
CHEMBL3237982 EC50 = 5670.0 nM 5.25
CHEMBL3238298 EC50 > 12500.0 nM -
CHEMBL3237990 EC50 > 12500.0 nM -
CHEMBL3237988 EC50 > 12500.0 nM -
CHEMBL3238315 EC50 > 12500.0 nM -
CHEMBL3238316 EC50 > 12500.0 nM -